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从金色庞巴诺Trachinotus ovatus中NOD1的功能性表征

Yadan Wang¹, Shaoyu Yang¹, Xiaohui Cai¹

¹Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, College of Marine Sciences, Beibu Gulf University, Qinzhou, 535011, China.

Fish & shellfish immunology

|April 18, 2024

概括

金色庞巴诺NOD1基因在鱼类免疫力中起着关键作用. 这种核酸结合的寡合化域类受体识别细菌成分并激活免疫通路,增强水产养殖中的抗病能力.

科学领域:

免疫学免疫学免疫学
水产养殖是水产养殖的一种方式.
分子生物学分子生物学

背景情况:

鱼类的先天免疫依赖于模式识别受体,如核酸结合的寡合化域类受体 (NLR).
金色 (Trachinotus ovatus) 是一个重要的水产养殖物种,需要对其免疫机制进行研究.

研究的目的:

为了克隆和表征来自金色庞巴诺 (Trachinotus ovatus) 的NOD1基因.
调查T. ovatus NOD1.1.的表达,配体识别和信号通路激活.

主要方法:

克隆和测序T. ovatus的基因 NOD1.1.
遗传学分析以确定进化关系.
通过细菌挑战试验进行基因表达分析.
配体识别测试和NF-κB信号通路激活研究.

主要成果:

T. ovatus NOD1 基因 (2820 bp) 编码了一种 939 氨基酸蛋白质,具有保存的 CARD-NACHT-LRR 域.
遗传学分析将T. ovatus NOD1放在鱼类中,但与其鸟类和哺乳动物对应物不同.
NOD1具有广泛的组织分布,在上表达最高,并受到细菌挑战 (S. agalactiae,V. alginolyticus) 的上调.
T. ovatus NOD1 识别了iE-DAP (≥100 ng/mL) 并激活了NF-κB信号通路.

结论:

证实NOD1对黄金的先天免疫反应至关重要.
这项研究有助于更好地了解在远程免疫中NOD1的功能,特别是在T. ovatus中.

关键词:

功能分析是一种功能分析.这是天生的免疫力.在NOD1中,NOD1是NOD1.它们中的一个是Trachinotus ovatus.

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