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一个灵敏和多功能的电化学传感器,基于杂交连锁反应和CRISPR/Cas12a系统进行抗生素检测

Xuemei Zhang¹, Li Zhu¹, Li Yang¹

¹Key Laboratory of the Evaluation and Monitoring of Southwest Land Resources (Ministry of Education), Sichuan Normal University, Chengdu, 610066, China; Sichuan Provincial Engineering Laboratory of Livestock Manure Treatment and Recycling (Sichuan Normal University), Chengdu, 610066, China; College of Chemistry and Materials Science, Sichuan Normal University, Chengdu, 610066, China.

Analytica chimica acta

|April 18, 2024

概括

这项研究介绍了一种新的电化学传感器,用于检测诸如卡纳米辛和安皮西林等抗生素. 该系统利用CRISPR/Cas12a和杂交连锁反应在食品和环境样本中进行高度敏感的检测.

科学领域:

电化学电化学电化学
分子生物学分子生物学
材料科学材料科学材料科学

背景情况:

食品和环境中的抗生素残留物构成重大风险.
对抗生素的敏感和快速检测方法对于公共卫生和环境监测至关重要.
现有的检测方法经常面临敏感性,特异性或样本矩阵干扰方面的挑战.

研究的目的:

开发一种敏感的电化学平台,用于抗生素检测.
整合CRISPR/Cas12a和杂交连锁反应 (HCR),以提高传感能力.
在牛奶和废水等现实世界样本中证明平台的有效性.

主要方法:

使用NH2-Cu-MOF作为探针的电化学平台的建造.
实施双重HCR系统 (HCR1和HCR2) 用于信号放大和目标识别.
使用CRISPR/Cas12a系统在目标检测时触发HCR1的分裂.
通过测量电化学信号变化来量化抗生素.

主要成果:

实现了对卡纳米辛 (60 fM) 和安皮西林 (10 fM) 的高度敏感检测.
显示了与抗生素度成比例的显著信号变化.
在复杂的矩阵中验证了平台的性能,例如牛奶和牲畜废水.
该CRISPR/Cas12a系统有效调节了HCR1过程,导致信号放大.

结论:

开发的电化学传感平台为抗生素检测提供了灵敏,快速和强大的方法.
结合NH2-Cu-MOF,CRISPR/Cas12a和HCR,为监测抗生素污染提供了一个强大的工具.
这项技术在食品安全和环境监测方面的应用非常有前途.

关键词:

这就是CRISPR/Cas12a.级联放大器的级联放大器电化学传感器是一种电化学传感器.杂交的连锁反应反应.NH (((2) Cu-MOFF 的使用情况.

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