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Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

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In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
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Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
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Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
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三种离子移动技术之间的跨平台比较,用于人体血脂质碰撞截面.

Anaïs C George1, Isabelle Schmitz1, Florent Rouvière2

  • 1Univ Rouen Normandie, INSA Rouen Normandie, CNRS, Normandie Univ, COBRA UMR 6014, INC3M FR 3038, F-76000, Rouen, France.

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概括

离子移动光谱 (IMS) 通过提供碰撞横截面 (CCS) 数据来增强代谢学. 这项研究表明,CCS值在不同的脂质IMS仪器中具有很高的可比性,使得可靠的分子描述.

关键词:
碰撞横截面的截面是碰撞的.漂流管离子移动性光谱学漂流管离子移动性光谱学实验室间的比较.利皮多米克 (Lipidomics) 是一种消化剂.捕获的离子移动性光谱法移动波离子移动性光谱学旅行波离子移动性光谱学

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科学领域:

  • 分析化学 分析化学
  • 代谢学 代谢学 代谢学
  • 频谱测量是一种光谱测量.

背景情况:

  • 离子移动光谱法 (IMS) 与液体染色学高分辨率质谱法 (LC-HRMS) 结合,改善了代谢学化合物注释.
  • 碰撞截面 (CCS) 是一个有价值的分子描述符,但缺乏用于其确定的标准化协议.
  • 关于CCS的跨平台协议存在有限的研究,特别是对于复杂的生物样本,如血脂质.

研究的目的:

  • 为了比较三种不同的离子移动技术 (DTIMS,TWIMS,TIMS) 中大约100种脂质物种的碰撞横截面 (CCS) 值.
  • 评估CCS测量的仪器可重复性和实验室间可重复性.
  • 评估CCS作为人类血脂质可靠分子描述物的潜力.

主要方法:

  • 使用漂流管IMS (DTIMS),移动波IMS (TWIMS) 和被困IMS (TIMS) 的商业可用和人体血提取脂质的CCS值的比较.
  • 评估11个CCS校准器,包括ESI低度调音混合 (Tune Mix).
  • 分析数据处理软件对CCS差异的影响,评估平台间的相关性和可重复性.

主要成果:

  • 观察到平均0.3%的CCS差异,受数据处理软件的影响.
  • 在所有仪器和离子模式中,ESI低度调整混合 (Tune Mix) 显示了CCS校准的最低相对标准偏差 (<0.5%).
  • 超过90%的脂质CCS值在使用Tune Mix校准时显示技术之间的偏差小于1%,具有高相关性 (R2>0.995) 和可重复性 (平均RSD ≤1%).

结论:

  • 对人体血脂类的CCS测定具有很高的可比性和可重复性,可以在不同的IMS技术中实现.
  • 校准,数据采集和处理的标准化程序对于跨平台可重现性至关重要.
  • 协调的分析条件对于可靠的CCS确定及其在代谢学中作为分子描述剂的使用至关重要.