通过-纳米通道-阵列-膜集成微流体芯片和抗体条形码生物芯片进行高效的EV分离和检测
Jiaoyan Qiu1, Qindong Guo2, Yujin Chu1
1Institute of Marine Science and Technology, Shandong University, Qingdao, Shandong, 266237, China.
Analytica chimica acta
|April 18, 2024
概括
这项研究引入了一种新型的微流体装置,用于有效地隔离和检测体液中的细胞外囊泡 (EV). 自动化芯片提供高通量分析,对于早期癌症诊断和临床研究至关重要.
科学领域:
- 生物标志物发现发现
- 纳米技术纳米技术
- 微流体学 微流体学
背景情况:
- 细胞外囊泡 (EVs) 是癌症早期检测的关键生物标志物.
- 目前的EV隔离和检测方法耗时,通量低,需要大量的样本.
- 从生物流体中有效,高通量隔离和检测EV仍然是一个重大挑战.
研究的目的:
- 开发一个具有成本效益的,自动化的微流体装置,用于高效的EV过和现场检测.
- 为了解决临床应用中传统的EV分析方法的局限性.
主要方法:
- 一个纳米尺寸的微流体装置,利用化纳米通道阵列膜进行EV过.
- 为提高过效率,优化了室阵列和受水性处理的通道直径 (90 nm).
- 集成多通道微流体生物芯片,用于现场EV捕获和基于抗体的表型监测.
主要成果:
- 使用优化的90nm化纳米通道膜,在没有化学或物理辅助的情况下,达到高达82%的过效率.
- 证明了高通量过 (几十个μL样本) 和识别不同的EV表型.
- 从临床血清和脑脊液样本中成功地进行了EV的实际过和检测.
结论:
- 开发的双功能集成芯片平台为电动汽车的分离和检测提供了具有成本效益,高效率和自动化解决方案.
- 该设备可直接分离和在现场检测EVs从血清或脑脊液82%的效率.
- 这种微流体装置在临床环境中为高效的EV识别和分析提供了巨大的潜力.
更多相关视频
06:12Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
Published on: March 17, 2023
1.4K
09:28Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery
Published on: October 27, 2023
2.9K
相关概念视频
01:15High-Performance Liquid Chromatography: Types of Detectors
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...
01:20Capillary Electrophoresis: Instrumentation
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
01:30Capillary Electrophoresis: Applications
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...