在TMTpro标签中克服有害的O-化,可以提高蛋白质组深度和量化精度
Yan Cai1, Chenchen Chang1, Rijing Liao1
1Shanghai Institute of Precision Medicine, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200125, China.
Analytica chimica acta
|April 18, 2024
概括
一种用于Tandem Mass Tag (TMTpro) 标签的新方法通过防止过度标签,显著改善了蛋白质组分析. 这种具有成本效益的方法可以提高蛋白质量化的深度和精度,从而在复杂的生物样本中获得更准确的结果.
科学领域:
- 蛋白质组学是指蛋白质组学
- 质谱测量质量谱测量
- 生物化学 生物化学
背景情况:
- 协同质量标记 (TMTpro) 试剂扩大样本复合,但导致含基残留的有害O-化.
- 这种过度标记,特别是与histidine,损害了蛋白质组分析的深度和精度.
- 现有的方法对含有基和基的具有有限的改善.
研究的目的:
- 开发一种新的TMTpro标签方法,克服有害的O-化.
- 为了提高标签效率并减少试剂的使用.
- 提高蛋白质组分析的深度和定量精度.
主要方法:
- 开发了一种新的TMTpro标签协议.
- 该方法使用酵母/人类双蛋白质组模型样本进行了验证.
- 与标准性标签方法与降低TMTpro试剂度的标准性标签方法的性能比较.
主要成果:
- 这种新方法在减少TMTpro试剂量的情况下实现了高标签效率.
- 检测到23.7%更多的独特和8.7%更多的蛋白质组,与性方法相比,在蛋白质深度>10,000.
- 显著提高了定量精度和蛋白质序列覆盖率,增加了差异蛋白质检测的统计能力.
结论:
- 开发的TMTpro标签方法有效地克服了有害的O-化.
- 这种新的方法大大提高了蛋白质组分析的深度,精度和统计能力.
- 该方法具有成本效益,并且与现有协议相比,提供了更好的结果.
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