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使用基于纳米孔测序的元基因组学,全面识别食品中的致病微生物和抗微生物耐药性基因
Annie Wing-Tung Lee1, Iain Chi-Fung Ng1, Evelyn Yin-Kwan Wong1
1Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong Special Administrative Region, China.
Food microbiology
|April 18, 2024
概括
对260种食品进行纳米孔测序,确定了关键的食源性病原体和抗菌素耐药性基因 (ARG). 新鲜,剥皮和碎的食物显示病原体水平较高,影响了食品安全评估.
科学领域:
- 食品安全微生物学 食品安全微生物学
- 转基因组学是指转基因组学.
- 抗微生物耐药性 (AMR) 是一种
背景情况:
- 食物传播的病原体和AMR细菌对全球健康构成重大风险.
- 检测病原体的传统培养方法缓慢且范围有限.
- 大基因组测序提供了一种更快,更全面的方法来识别微生物和AMR基因 (ARG).
研究的目的:
- 调查食品类型和属性的对微生物群落和AMR概况的影响.
- 为了将纳米孔长读序列与细菌分类的短读序列进行比较.
- 评估各种食品中食源性病原体和ARG的流行程度.
主要方法:
- 在260个食品样本 (生肉,沙希米,RTE蔬菜) 上利用纳米孔长读元基因组测序.
- 分析了食品原产地,零售地点和加工方法对微生物组成和AMR的影响.
- 在分析的食品矩阵中发现了特定的食源性病原体和ARG.
主要成果:
- 确定了Clostridium botulinum,Acinetobacter baumannii和Vibrio parahaemolyticus作为生肉和沙希米中普遍存在的病原体.
- 在肉和RTE蔬菜中分别检测到较高的碳烯和氨酸耐药基因的患病率,即使病原体水平较低.
- 发现新鲜,剥皮和碎的食物含有更高水平的致病细菌.
结论:
- 纳米孔元基因组测序为食品微生物群落和AMR提供了准确和全面的见解.
- 食品类型和加工方式显著影响病原体和ARG概况,对食品安全有影响.
- 这项研究为加强食品安全评估和建立质量指标提供了有价值的数据.
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