在黄发酵过程中,细菌群体的继承和纯素化合物变化之间的相关性
Xianglin Wang1, Guolin Cai2, Dianhui Wu3
1The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, China; National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, China; Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi, 214122, China.
Food microbiology
|April 18, 2024
概括
黄江的纯素水平在早期发酵期间增加,与特定的乳酸细菌 (LAB) 有关. 了解这些细菌群落有助于控制精氨酸含量,减少导致痛风的化合物.
科学领域:
- 食品微生物学 食品微生物学
- 发酵科学是发酵的科学.
- 生物化学 生物化学
背景情况:
- purin 是高尿血和痛风的主要原因.
- 传统的中国葡萄酒黄酒含有纯素作为自由基.
- 细菌的继承对黄江的质量至关重要,但其与纯素含量的联系尚未被探索.
研究的目的:
- 调查黄江发酵过程中细菌群落和纯素化合物水平之间的相关性.
- 为了确定关键的细菌物种和参与清素形成的代谢途径.
- 为了提供一个科学基础来调节黄江州的纯素水平.
主要方法:
- 高通量测序 (HTS) 用于分析细菌社区动态.
- 斯皮尔曼分析以将细菌群体与物理化学性质和纯素含量相关联.
- 微生物纯素代谢中的关键酶的生物信息分析 (PICRUSt).
主要成果:
- 纯素含量从第0天到第9天显著增加 (21.05-65.71毫克/升),从第12天到第18天 (65.63-69.55毫克/升) 稳定下来.
- 乳酸细菌 (LAB) 的大量反映了这一趋势,增加,然后稳定.
- 乳酸菌酸耐受性和乳酸菌 helveticus与纯素含量呈正相关性,而Limosilactobacillus fermentum和乳酸菌植物体则显示出负相关性.
- 细菌纯素合成酶比降解酶更为丰富.
结论:
- 细菌的继承显著影响黄州的氨酸积累.
- 特定的LAB菌株,如L. acetotolerans和L. helveticus,驱动 purin的增加.
- 这些发现支持有针对性的菌株选择,以控制纯素水平,并减轻与黄江消费相关的HUA/痛风风险.
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