防真菌效率的DBD冷血对阿斯伯吉路斯尼格尔在干燥的朱果的杀菌效率
Qiaoyun Wei1, Yuan Yuan2, Jianhao Zhang1
1National Center Meat Quality & Safety and Control, College of Food Science & Technology, Nanjing Agricultural University, Nanjing 210095, China; Nanjing Suman Plasma Engineering Institute Co. LTD, Nanjing 210095, China.
Food microbiology
|April 18, 2024
概括
介电屏障放电冷气氛等离子体 (DBD-CAP) 通过破坏细胞膜,有效地使Aspergillus niger子失活. 这种方法可以显著降低干的真菌负荷,而不会影响质量.
科学领域:
- 食品微生物学 食品微生物学
- 激光等离子技术技术
- 生物物理科学 生物物理科学
背景情况:
- 黑色菌对食品产品构成显著的腐败风险.
- 有效且无害的灭菌方法对于食品的保存至关重要.
- 冷大气等离子体 (CAP) 是微生物无活化的新兴技术.
研究的目的:
- 为了评估介电屏障放电冷气氛等离子体 (DBD-CAP) 对黑色阿斯伯吉路斯子的杀菌效果.
- 通过DBD-CAP阐明子无活化的机制.
- 评估DBD-CAP处理对干的质量的影响.
主要方法:
- 使用韦布尔+尾巴模型建模了真菌子无活化动力学.
- 微生物无活化通过日志减少量化 (cfu/mL和cfu/g).
- 使用扫描电子显微镜 (SEM) 和传输电子显微镜 (TEM) 观察到微结构变化.
- 通过测量相对导电性和核酸/蛋白质泄漏来评估细胞膜完整性.
- 分析了经过处理的干燥朱的质量属性.
主要成果:
- 在15分钟后,DBD-CAP在70kV的悬浮中实现了4.05 log (cfu/mL) 的A. niger子减少.
- 在SEM和TEM中,治疗子中发现了细胞膜损伤和细胞内泄漏.
- 活性氧物种 (ROS) 的增加与细胞导电率升高和核酸/蛋白质泄漏相关.
- 幸存子的生长和毒性显著减少.
- 在没有显著的质量退化 (P > 0.05) 的干果上,可以实现2.67 log cfu/g的子减少.
结论:
- DBD-CAP是一种高效的方法,可以使Aspergillus niger子失活.
- 主要机制涉及等离子体诱导的细胞膜损伤和氧化应激.
- DBD-CAP提供了一种有前途的非热污染策略,用于干燥的朱,保持产品质量.
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