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巴西手工奶酪中的中毒性黄金葡萄球菌:发生率,数量,表型和基因型概况
Larissa P Margalho1, Juliana S Graça1, Bruna A Kamimura1
1Department of Food Science and Nutrition, Faculty of Food Engineering, University of Campinas, Campinas, SP, Brazil.
Food microbiology
|April 18, 2024
概括
这项研究在巴西手工奶酪中发现了高水平的金黄色葡萄球菌,许多菌株对消毒剂有抗性,并携带毒素基因. 这对公众健康构成重大风险,需要提高奶酪生产的卫生水平.
科学领域:
- 食品微生物学 食品微生物学
- 食品安全 食品安全
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 工艺奶酪很受欢迎,但可以庇护食物传播的病原体.
- 黄金葡萄球菌 (Staphylococcus aureus) 是导致食物传播疾病的一个重要原因.
- 了解其在巴西手工奶酪中的流行程度和特征对于公共卫生至关重要.
研究的目的:
- 评估巴西手工奶酪 (BAC) 中黄金葡萄球菌的发生率和数量.
- 评估S. aureus菌株对消毒剂的耐药性及其粘附于表面的能力.
- 确定从BAC. aureus中分离的S. aureus中肠毒基因和抗菌素耐药性概况的流行率.
主要方法:
- 来自巴西五个地区的BAC采样.
- 定量化S. aureus的数量 (日志CFU/g) 和流行率 (%).
- 对低化 (200 ppm) 的耐药性的评估.
- 在不钢上评估生物膜的形成.
- 抗生素分析和检测肠毒素基因 (sea-ser).
- 脉冲场凝电泳 (PFGE) 用于菌株类型.
主要成果:
- 在BAC.中发现了高S. aureus数量 (3.4-6.4log CFU/g) 和流行率 (25-62.5%).
- 16%的S. aureus菌株抵抗了200ppm的二酸盐.
- 87.6%的菌株对不钢表面表现出强烈的附着性.
- 80.5%的分离物是多药耐药的.
- 进入毒素基因在92.6%的评估菌株中存在.
结论:
- 巴西手工奶酪中含有相当多的金黄色葡萄球菌 (Staphylococcus aureus).
- 多抗药性和肠毒性黄金菌株的存在构成了公共卫生风险.
- 加强卫生实践,动物福利和控制污染源对于改善BAC的微生物质量和安全至关重要.
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