[肠道微生物群及其代谢物调节失调和老年男性C57BL/6J小鼠手术后认知功能障碍之间的关系]
Y Z Cheng1, X R Lian2, H X Li1
1Department of Anesthesiology, National Cancer Center, National Clinical Research Center for Cancer, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
Zhonghua yi xue za zhi
|April 18, 2024
概括
手术和麻醉破坏了肠道微生物群及其代谢物,导致老年小鼠的术后认知功能障碍. 这些变化会影响肠道细菌和代谢特征,影响认知功能.
科学领域:
- 微生物组研究的研究.
- 神经科学是一个神经科学.
- 代谢学 代谢学 代谢学
背景情况:
- 手术后认知功能障碍 (POCD) 是老年患者的一个重大问题.
- 肠道微生物群及其代谢产物在维持生理恒温中起着至关重要的作用.
- 新出现的证据表明,肠道微生物组与神经功能之间存在联系.
研究的目的:
- 在老年雄性小鼠中调查肠道微生物群失调和POCD之间的关系.
- 分析探索性腹腔切除术和麻醉对肠道微生物组成和便代谢学的影响.
- 探索POCD与肠道微生物组改变相关的潜在生物标志物.
主要方法:
- 老年雄性C57BL/6J小鼠在异氨酸麻醉下进行了探索性腹腔切除术,或作为对照.
- 用16S rDNA基因测序分析了肠道微生物组成.
- 通过液体染色学-并联质谱法 (LC/MS) 评估便代谢概况.
主要成果:
- 在莫里斯水迷宫测试中,手术小鼠表现出延长的逃脱延迟和减少目标象限居住时间,表明POCD.
- 观察到肠道微生物群的显著变化,大肠杆菌,西格拉菌和杆菌的增加,鲁米诺科克和布蒂里西科克的减少.
- LC/MS分析揭示了便样本中的差异性代谢物,主要影响氨基酸,脂肪酸和氨酸代谢途径.
结论:
- 肠道微生物群及其代谢物的失调与老年小鼠中POCD的发展有关.
- 麻醉和手术显著改变肠道细菌结构和代谢平衡.
- 这些发现表明肠道微生物组是缓解POCD的潜在治疗点.
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