在全球肠道多中心研究中,使用定量分子测试检测出肠道病毒的临床严重性,与传统测试相比
Jordan Cates1, Helen Powell2, James Platts-Mills3
1Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
The Journal of infectious diseases
|April 18, 2024
概括
与传统方法相比,定量分子测试可能会改变肠道病毒感染的感知严重程度,特别是轮状病毒. 仔细考虑周期值的截止值对于研究中的准确解释至关重要.
科学领域:
- 病毒学 病毒学
- 临床微生物学 临床微生物学
- 儿童传染病 儿童传染病
背景情况:
- 定量分子检测越来越多地用于检测肠道病毒.
- 传统的诊断方法包括酶免疫测试 (EIA) 和聚合酶链反应 (PCR).
研究的目的:
- 为了比较通过常规测试诊断的肠道病毒感染的临床严重程度与定量分子测试.
- 评估周期值 (Ct) 切断对量化分析中的病例严重性的影响.
主要方法:
- 该研究比较了0-59个月龄的儿童肠道病毒的临床严重程度 (修改后的Vesikari得分).
- 罗塔病毒和腺病毒病例基于EIA和TaqMan阵列卡 (TAC) 结果进行了分析,对TAC阳性,EIA阴性病例的Ct截止值有所不同.
主要成果:
- 通过常规测试检测到的罗塔病毒病例显示出更高的临床严重性中位数.
- 有较低Ct值的EIA阴性/TAC阳性轮状病毒病例的严重性得分明显较低.
- 腺病毒40/41的严重程度在不同Ct截止点的EIA阳性和TAC阳性病例之间是可比的.
结论:
- 定量分子检测可能会影响评估肠道病毒病例严重程度,特别是轮状病毒.
- 建立适当的周期值截止值对于精确解释肠道病毒研究中的定量分子检测结果至关重要.
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