相关实验视频

Updated: Jun 28, 2025

Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles

Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles

Published on: May 14, 2021

与癌症相关的纤维细胞分泌FGF5,以抑制铁亡,通过结合FGFR2降低鼻癌中西斯普拉丁的敏感性

Feng Liu¹, Ling Tang¹, Huai Liu¹

¹Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, 410013, Hunan Province, P. R. China.

Cell death & disease

|April 18, 2024

概括

与癌症相关的纤维细胞通过分泌FGF5来抑制鼻癌 (NPC) 中的西斯普拉丁诱导的铁化,从而降低了治疗的敏感性. 向FGF5/FGFR2通路可以提高思丁的疗效,并减少NPC患者的副作用.

科学领域:

在瘤学瘤学.
癌症生物学癌症生物学
细胞信号传输细胞信号传输

背景情况:

基于西斯普拉丁 (DDP) 的化学放射治疗是一种标准的鼻癌 (NPC) 治疗方法.
DDP敏感性和副作用是NPC治疗中的重大挑战.
癌症相关纤维细胞 (CAFs) 在DDP耐药性中的作用尚未完全理解.

研究的目的:

研究CAF如何通过ferroptosis调节NPC中的DDP敏感性.
阐明DDP诱导的铁灭的CAF介导调节背后的分子机制.

主要方法:

使用NPC细胞系和CAF进行体外研究.
在小鼠体内异种移植模型.
对ferroptosis诱导,FGF5分泌,FGFR2结合和Keap1/Nrf2/HO-1信号通路激活的分析.

主要成果:

DDP诱导了NPC细胞中的铁亡,并在体内抑制了瘤的生长.
CAFs分泌FGF5,这抑制了NPC细胞中的DDP诱导的铁亡.
来自CAF的FGF5与NPC细胞中的FGFR2结合,激活Keap1/Nrf2/HO-1通路.
通过FGF5/FGFR2轴,FGFR2过度表达和CAF保护了NPC细胞免受DDP诱导的铁亡.

结论:

通过FGF5/FGFR2/Nrf2信号通路抑制铁亡,CAFs降低了NPC中的DDP敏感性.
针对FGF5/FGFR2轴是一个潜在的治疗策略,可以提高DDP的敏感性并减少NPC治疗中的副作用.

更多相关视频

Isolation of Normal and Cancer-associated Fibroblasts from Fresh Tissues by Fluorescence Activated Cell Sorting FACS

Isolation of Normal and Cancer-associated Fibroblasts from Fresh Tissues by Fluorescence Activated Cell Sorting FACS

Published on: January 14, 2013

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

Published on: March 15, 2024

相关概念视频

Mitogens and the Cell Cycle

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...

Introduction to Fibroblasts

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...