1型分泌需要ABC载体的所有域之间紧密的相互作用
Manuel T Anlauf1, Florestan L Bilsing1, Jens Reiners2
1Institute of Biochemistry, Heinrich Heine University Düsseldorf, Universitätsstraße 1, 40225, Düsseldorf, Germany.
Scientific reports
|April 18, 2024
概括
血解素T1SS载体HlyB的C端酶样域 (CLD) 对于基质分泌至关重要. 交换域名显示了CLD.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- I型分泌系统 (T1SS) 对于格拉姆阴性细菌在细胞外中输出蛋白质至关重要.
- 大肠杆菌中的血清素分泌系统 (T1SS),涉及HlyB,HlyD和TolC,分泌毒素HlyA.
- HlyB是一种ABC载体,包括C39酶类域 (CLD),跨膜域 (TMD) 和核酸结合域 (NBD).
研究的目的:
- 为了研究HlyB传送器内个别域的功能作用.
- 确定HlyB的CLD,TMD和NBD对HlyA分泌的贡献.
- 通过使用模拟结构,探索ABC传送器中的基质识别机制.
主要方法:
- 通过域互换与来自Kingella kingae的RtxB构建模拟HlyB传送器.
- 化学载体与HlyD的共同表达以及对亲HlyA分泌的评估.
- 对不同域组合的分泌效率进行比较分析.
主要成果:
- 完全替代CLD取消了亲HlyA分泌,突出了它的关键作用.
- 部分交换TMD或NBD降低了分泌效率.
- 同时交换TMD和NBD,或所有三个域,也取消了分泌.
结论:
- 在HlyB T1SS中,CLD是基质识别和分泌的主要决定因素.
- TMD和NBD扮演了次要的角色,可能涉及额外的交互站点或监管.
- 最佳分泌需要所有HlyB域之间的协调功能和相互作用,这代表了ABC载体的不寻常的基质识别策略.
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