短链脂肪酸产生细菌菌株通过通过JAK/STAT3/FOXO3轴不活化促进M2巨分化来减弱实验性性结肠炎
Hailan Zhao1,2,3, Youlian Zhou1, Jing Xu1
1Department of Gastroenterology and Hepatology, Guangzhou First People's Hospital, the Second Affiliated Hospital, School of Medicine, South China University of Technology, Guangzhou, 510006, China.
七种短链脂肪酸 (SCFA) 生产细菌及其培养超物的混合物有效地治疗了小鼠的实验性性结肠炎 (UC),为便微生物群移植 (FMT) 提供了更安全的替代方案. 这些治疗方法通过调节巨细胞极化来减少炎症和改善肠道屏障功能.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 患有炎症性肠病 (IBD),失生症和免疫抑制的患者面临便微生物群移植 (FMT) 的风险.
- 短链脂肪酸 (SCFA) 生产细菌对肠道健康至关重要,在性结肠炎 (UC) 中观察到丰度下降.
研究的目的:
- 为了研究七个菌株SCFA产生细菌混合物 (7-mix) 和其培养超剂 (mix-sup) 在治疗实验性UC的疗效.
- 将这些干预措施与FMT进行比较,并阐明它们的潜在机制.
主要方法:
- 从健康的供体中分离和培养SCFA产生细菌.
- 在小鼠中使用硫酸 (DSS) 诱导急性UC.
- 使用各种技术评估7-mix,mix-sup和FMT对炎症,屏障功能,微生物组成和巨细胞两极分化的影响.
主要成果:
- 在UC小鼠中,7-mix和mix-sup都在抑制炎症和增强肠道屏障功能方面表现出与FMT相似或优于FMT的疗效.
- 这些干预措施增加了SCFA产生细菌和SCFA度在结肠病小鼠.
- 治疗效果是由巨细胞中JAK/STAT3/FOXO3轴的失活介导的,促进M2极化.
结论:
- 开发的SCFA产生细菌混合物及其超剂为IBD治疗提供了FMT有希望的,可能更安全的替代品.
- 这种方法促进了基于益生菌和代谢物的合理开发疗法,以减少肠道炎症和恢复肠道微生物平衡.
更多相关视频
06:57Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells
Published on: September 18, 2016
08:58Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis
Published on: January 5, 2017
相关概念视频
Drugs for Treatment of Ulcerative Colitis in IBD
Pathophysiology of Peptic Ulcer Disease: Mucosal Defense Factors
Inflammatory Bowel Disease I: Ulcerative Colitis
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
