Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Affinity Chromatography01:03

Affinity Chromatography

632
Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
632

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Unveiling the molecular crosstalk between the purinergic P2X7 receptor and cannabinoid subtype 2 receptor in human microglial HMC3 cells: New insights for multitarget therapies in neuroinflammation.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Discovery of bridged melanostatin derivatives as potent allosteric modulators of the dopamine D<sub>2</sub> receptors with improved permeability.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Synthesis and Biological Profiling of New 1,2,3,4-Tetrahydrobenzo[<i>h</i>]naphthyridine-Based Hybrids as Dual Inhibitors of β-Amyloid and Tau Aggregation with Anticholinesterase Activity.

Biomolecules·2026
Same author

Buffers in intravenous solutions: is the source of bicarbonate a source of confusion?

Critical care (London, England)·2025
Same author

Distinctive patterns of G protein coupling induced by the structurally similar 5-HT<sub>2A</sub> receptor ligands ketanserin and altanserin in human prefrontal cortex.

Molecular pharmacology·2025
Same author

In Silico and In Vivo Pharmacological Evaluation of Iridoid Compounds: Geniposide and Asperuloside Profile Study Through Molecular Docking Assay and in the <i>Caenorhabditis elegans</i> Model.

Biomolecules·2025

相关实验视频

Updated: Jun 28, 2025

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
10:17

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

7.8K

高通量DNA编码图书馆 关联性选择平台用于用固体支蛋白固定固定结合物识别.

Ramón Rama-Garda1,2, Eduardo Domínguez3, María Isabel Loza2

  • 1Discovery Chemistry Research & Technologies, Lilly Research Laboratories, Eli Lilly and Company, Alcobendas, Spain.

Assay and drug development technologies
|April 19, 2024
PubMed
概括
此摘要是机器生成的。

一个新的自动化平台增强了DNA编码库 (DEL) 选用于连接体识别. 这种高通量系统提高了药物发现研究的亲和性选择 (AS) 的可重现性和灵活性.

关键词:
用DNA编码的图书馆.亲和关系的选择 亲和关系的选择自动化自动化自动化自动化质量控制质量控制质量控制

更多相关视频

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates
13:49

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

Published on: December 6, 2017

11.4K
Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

24.8K

相关实验视频

Last Updated: Jun 28, 2025

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
10:17

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

7.8K
Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates
13:49

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

Published on: December 6, 2017

11.4K
Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

24.8K

科学领域:

  • 生物化学 生化学
  • 化学生物学 化学生物学
  • 药物发现 药物发现 药物发现

背景情况:

  • DNA编码库 (DEL) 是识别新型连接体的强大工具.
  • 亲和选择 (AS) 是DEL中用于寻找结合物的核心方法.
  • 当前的AS方法在可重现性和吞吐量方面可能面临挑战.

研究的目的:

  • 为DNA编码库 (DEL) 开发一个高通量,自动化亲和关系选择 (AS) 平台.
  • 提高AS过程在配体识别中的可重复性和灵活性.
  • 为DEL应用程序创建一个与工业标准兼容的平台.

主要方法:

  • 开发一个完全自动化的平台,用于高通量亲和性选择.
  • 实施了两个主要的蛋白质固定技术:磁珠和树脂尖端柱.
  • 可配置的协议选项支持多种DEL类型和96个同步样本的实验条件.

主要成果:

  • 该平台展示了高吞吐量和灵活性,可以容纳各种DEL和AS方法.
  • 它支持磁珠和树脂尖柱固定蛋白质.
  • 该系统允许进行常规的DEL屏幕,共价图书馆选和蛋白质固定验证.

结论:

  • 自动化AS平台显著提高了DEL绑定器识别的可重复性.
  • 它的多功能性使其适用于学术和工业药物发现中的广泛应用.
  • 这项技术提供了一种强大的工具,用于使用DELs推进配体识别.