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在基于染料的纳米粒子中控制光寿命

Stine G Stenspil¹, Junsheng Chen¹, Mikkel B Liisberg¹

¹Nano-Science Center & Department of Chemistry, University of Copenhagen Universitetsparken 5 2100 København Ø Denmark bwl@chem.ku.dk.

Chemical science

|April 19, 2024

概括

研究人员开发了新的光染料纳米粒子 (NP) 用于时间分辨率成像. 这些NP克服了终身信号问题,实现了增强的多色成像和诊断,并改进了信号与背景比率.

科学领域:

纳米技术纳米技术
光成像技术光成像技术
频谱学是一种光谱学.

背景情况:

光染料纳米粒子 (NP) 为成像提供亮度和稳定性.
时间分辨率成像增强了对背景的信号,并将重叠的信号分开.
结合基于染料的NP与时间分辨率成像是具有挑战性的,因为由于聚合引起的火 (ACQ) 和能量迁移导致的缩短,非单指数寿命.

研究的目的:

开发与时间分辨率成像相容的基于染料的纳米粒子.
为了克服基于染料的NP的光寿命信号质量的局限性.
为了使多色成像使用光谱相同但时间不同的纳米粒子.

主要方法:

利用小分子离子隔离格子 (SMILES) 概念来防止ACQ.
集成的Förster共振能量转移 (FRET) 染料对来控制光寿命.
制造的SMILESNP与氨酸供体染料和罗达胺或氧三烯FRET接受器合.

主要成果:

实现了具有相同辐射光谱和高量子产量的NP,但具有不同的光寿命 (3 ns和26 ns).
通过光寿命成像,在单个粒子水平上成功区分光谱相同的NP.
通过兴奋剂展示了可预测的光寿命,使其能够用于时间解析检测.

结论:

SMILES方法有效地防止了ACQ,并使基于染料的NP能够控制光寿命.
杂的NP与时间分辨率成像相容,克服了以前的限制.
这一策略为先进的多色和时间分辨率应用提供明亮,稳定的成像试剂.

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