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在草中发现了一种内源性促进物LpSUT2:对于植物来说,这是一个有希望的转基因工具

Cuicui Wei^1,2, Zhubin Hu^1,2, Songhu Wang³

¹CAS Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.

Frontiers in plant science

|April 19, 2024

概括

花草马赛克病毒35S促进体在抗生素压力下减少外源蛋白质表达. 一种新型的草LpSUT2促进剂保持高活性,为植物基因工程提供了稳定的替代方案.

科学领域:

植物生物技术植物生物技术
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

促进者对于调节基因表达至关重要,并且是生物技术中用于异质蛋白质生产的必不可少的工具.
花草马赛克病毒35S促销剂在植物中广泛使用,但其在压力下的表现尚不清楚.

研究的目的:

为了研究抗生素应激对广泛使用的35S促进体的影响.
发现和描述在植物基因工程的压力条件下表达稳定的新型促进体.

主要方法:

用含有35S促进体和新型LpSUT2促进体的结构驱动eGFP表达的duckweed的稳定转化.
在抗生素G418的不同度下对eGFP基因表达和光的定量分析.
在抗生素压力下对促进物甲基化的分析.

主要成果:

在增加G418度下,35S促进体表达减少,这与促进体甲基化和基因沉默有关.
即使在高G418压力下,草LpSUT2促进剂也保持了高的eGFP表达和光水平.
在抗生素压力下,LpSUT2促进体活性显著高于35S促进体活性 (1.76x在100 mg/L G418,6.18x在500 mg/L G418).

结论:

LpSUT2促进剂是35S促进剂的强大而稳定的替代品,用于在抗生素压力下需要一致的基因表达的应用.
这项研究揭示了在压力下促进体甲基化诱导的沉默的新机制,并为选抗压性促进体提供了洞察力.
LpSUT2促进剂显示出在植物遗传工程和生物技术应用中使用的巨大潜力.

关键词:

35S 促销器的使用 LpSUT2 的促进者.抗生素压力抗生素压力草是一种草.内源性强促销剂是内源性的强促销剂.

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