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PsmiR159b-PsMYB65模块通过影响树的细胞循环来恢复芽生长后的味道
Tao Zhang1,2,3, Xinyu Wang1,2, Yanchao Yuan1,2
1College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, China.
Horticulture research
|April 19, 2024
概括
树中的芽休眠释放受PsmiR159b-PsMYB65模块的调节. 这一途径通过影响休眠后的细胞循环进展和增殖来促进芽.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 芽的气味是多年生植物对季节性气候变化的关键适应.
- 吉伯雷林 (GAs) 和低温是内臭释放 (EDR) 后芽破裂的关键信号.
- 在树中由GA介导的芽破裂的精确分子机制尚未完全理解.
研究的目的:
- 阐明miR159家族在树 (Paeonia suffruticosa) 中GA介导的芽中发挥的调节作用.
- 为了确定PsmiR159b的下游目标及其在芽休眠释放中的功能.
主要方法:
- 在冷却过程中进行基因表达分析 (包括RNA-seq) 来研究PsmiR159b和PsMYB65.
- 基因沉默和过度表达技术,以评估PsmiR159b和PsMYB65在芽中的功能.
- 酵母单杂交,电泳性移动性转移测定和双露西法酶记者测定以确认基因相互作用和调节效应.
主要成果:
- 随着冷却,PsmiR159b的表达量下降,其操纵影响了芽时间.
- 一个GA通路转录因子PsMYB65被冷却和GA3诱导,并被PsmiR159b准.
- PsMYB65直接激活了细胞周期基因PsCYCD3;1的表达,促进了细胞增殖和芽.
结论:
- PsmiR159b-PsMYB65模块在树休眠后的芽生长调节中发挥着至关重要的作用.
- 该模块通过调节细胞循环的进展和增殖来发挥作用,提供了对内臭释放机制的洞察.
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