在Clostridioides difficile挑战之后,小鼠,蒙古大鼠和仓鼠模型中的肠道微生物组变化
Shuangshuang Wan1,2, Peijun You1, Qikai Shi1
1School of Laboratory Medicine, Hangzhou Medical College, Hangzhou, China.
Frontiers in microbiology
|April 19, 2024
概括
蒙古鼠是研究Clostridioides difficile感染 (CDI) 的合适动物模型,表现出类似于人类的症状和肠道微生物组变化. 这项研究推进了CDI建模和理解相关的肠道失调.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 动物建模动物建模
背景情况:
- 艰难杆菌感染 (CDI) 研究受到反映人类症状和肠道微生物组变化的适当动物模型缺乏的阻碍.
- 现有的模型不能完全捕捉到CDI病原和宿主微生物群相互作用的复杂性.
研究的目的:
- 评价蒙古鹿作为一种新的动物模型,用于Clostridioides difficile感染 (CDI).
- 为了比较CDI诱导的蒙古鹿肠道微生物组变化与小鼠和仓鼠的变化.
主要方法:
- 蒙古子,老鼠和仓鼠感染了高毒的C. difficile菌株 (ribotype 027).
- 使用16S rRNA基因测序对肠道微生物组合的比较分析.
- 评估临床症状和肠道组织病理学.
主要成果:
- 蒙古小鼠表现出模仿人类CDI的腹和肠道病理,与没有显著变化的小鼠不同.
- 仓鼠表现出严重的肠道出血和炎症.
- 微生物组分析显示,不同模型中的Firmicutes/Bacteroidetes比率和Proteobacteria和Verrucomicrobia的丰富性有明显的变化,其中Lactobacillaceae和Akkermansia被确定为关键的区分分类.
结论:
- 蒙古鹿作为一个可行的动物模型来研究Clostridioides difficile感染 (CDI).
- 已识别的微生物种群可以作为CDI研究的生物标志物,并了解相关疾病的肠道微生物群变化.
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