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酵母Crf1p是一种激活剂,在调节基因的过程中具有不同的作用

Sanjay Kumar¹, Muneera Mashkoor¹, Priya Balamurugan¹

¹Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana, USA.

Yeast (Chichester, England)

|April 19, 2024

概括

在压力下,核糖体的产生会减缓. 研究人员发现,Crf1p充当弱激活剂,通过防止Ifh1p在酵母中的mTORC1抑制过程中重新结合,帮助控制基因表达.

科学领域:

分子生物学分子生物学
细胞生物学细胞生物学
酵母遗传学酵母遗传学

背景情况:

在压力下,核糖体生物发生是下调的.
机械目标的拉帕米辛复合物1 (mTORC1) 途径感知压力,并改变基因表达.
在Saccharomyces cerevisiae中,核糖体蛋白基因下调涉及Ifh1p解离,依赖于Utp22p.

研究的目的:

研究Utp22p和Hmo1p在传达mTORC1抑制中的作用.
澄清Crf1p的功能,Ifh1p的一个对应物,在调节核糖体生物发生基因.
确定 Crf1p 在 mTORC1 抑制过程中如何影响 Ifh1p 动态.

主要方法:

基因表达分析 (mRNA丰富度).
RNA聚合酶II占用率研究.
对基因删除菌株 (crf1Δ) 的分析.

主要成果:

Crf1p作为Utp22p和Hmo1p基因的激活剂,由crf1Δ菌株的mRNA和Pol II占用率降低所证明.
mTORC1抑制对HMO1和UTP22的表达有不同的影响.
在mTORC1抑制过程中,Crf1p促进了UTP22基因上的Ifh1p耗尽,但不是HMO1.

结论:

在调节核糖体生物发生基因方面,Crf1p作为弱激活剂起作用.
在mTORC1抑制后,Crf1p可能会阻止Ifh1p与促进体重新结合.
核糖体蛋白基因及其调节者的共同调节优化了对mTORC1抑制的转录反应.

关键词:

这就是Hmo1pp.如果h1pp.这就是Sfp1p.Utp22pp 在线阅读基因调节基因调节基因调节在 mTORC1 的情况下,mTORC1 是

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