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使用单颗粒ICP-TOF-MS对蛋白质功能化磁性微粒的结合量化

Veronica C Bradley¹, Benjamin T Manard¹, Lyndsey Hendriks²

¹Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA. manardbt@ornl.gov.

Analytical methods : advancing methods and applications

|April 19, 2024

概括

一种新的分析方法,单颗粒子诱导合等离子体飞行时间质谱法 (SP-ICP-TOF-MS),准确地测量了对微粒的结合效率. 这种技术可以区分结合与未结合的金,克服了批量消化方法的局限性.

科学领域:

分析化学分析化学
材料科学材料科学材料科学
纳米技术纳米技术

背景情况:

准确量化金属与微粒的结合对于诊断和治疗领域的应用至关重要.
传统的批量消化方法可能会因为未结合的分析物而高估金属负载.
蛋白质涂层的磁性微粒用于各种需要精确表征的生物医学应用.

研究的目的:

开发和验证单颗粒子诱导合等离子体飞行时间质谱法 (SP-ICP-TOF-MS) 方法,以确定的结合效率.
为了比较SP-ICP-TOF-MS的准确性与传统的散装消化诱导合等离子体质谱法 (ICP-MS) 进行量化.
评估蛋白质涂层磁性微粒上的负荷和颗粒-协会.

主要方法:

开发一种SP-ICP-TOF-MS分析程序,用于同时检测和铁.
使用批量消化-ICP-MS和SP-ICP-TOF-MS对1μm磁性微粒的表征.
量化每颗颗粒的负荷,并确定和颗粒的关联性,以 streptavidin 和 azurin 涂层的微粒.

主要成果:

通过SP-ICP-TOF-MS,几乎可以同时检测所有核化物,从而可以同时测量和铁.
从SP-ICP-TOF-MS和散装消化-ICP-MS的结果同意粒子特征,但SP-ICP-TOF-MS提供了准确的装载.
量化负荷为0.18 ± 0.02 fg/颗粒 (斯特雷普塔维丁) 和0.32 ± 0.02 fg/颗粒 (亚苏林),颗粒与的关联率高于65%.
大量ICP-MS高估了白金装载量,因为存在未绑定的白金.

结论:

与散装消化技术相比,SP-ICP-TOF-MS是一种优越的方法,可以准确量化对微粒的结合效率.
开发的方法消除了繁的样品准备,并允许区分绑定和未绑定的.
这种粒子对粒子量化方法对各种基于单个粒子的分析具有广泛的适用性.

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