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骨髓介质干细胞调节媒介对脂瘤瘤细胞的增殖和迁移的影响

Hua Chen¹, Na Sha², Ning Liu²

¹Department of Emergency, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, The First Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518020, Guangdong, China. chenhua88@163.com.

Folia histochemica et cytobiologica

|April 19, 2024

概括

人类骨髓中介细胞干细胞条件介质 (BMSC-CM) 促进脂肪瘤的生长. 然而,减少BMSC-CM中的YAP1抑制了脂肉瘤细胞的增殖和迁移,提供了潜在的治疗策略.

科学领域:

在瘤学瘤学.
干细胞生物学干细胞生物学
癌症研究癌症研究

背景情况:

脂质肉瘤是一种常见的软组织肉瘤,对化疗反应有限.
介酶干细胞 (MSCs) 可以影响瘤的进展.
人类骨髓介质干细胞 (BMSCs) 在脂肉瘤中的作用尚不清楚.

研究的目的:

研究BMSC受条件介质 (BMSC-CM) 对脂肉瘤细胞增殖和迁移的影响.
探索BMSC-CM行动的基本机制,重点关注YAP1.
评估YAP1在BMSC中对脂瘤瘤细胞的影响.

主要方法:

脂肉瘤细胞系 (93T449,SW872) 与BMSC-CM或来自YAP1-敲击BMSC (shYAP1-BMSC-CM) 的条件介质共同培养.
使用MTT测定来评估扩散.
对YAP1,Bcl-2和MMP-2的亡,迁移和表达被通过流细胞计和西布洛特评估.

主要成果:

BMSC-CM增强了脂肉瘤细胞的增殖.
shYAP1-BMSC-CM显著抑制了细胞活力和迁移.
shYAP1-BMSC-CM诱导的亡和下调的Bcl-2和MMP-2表达.

结论:

BMSC-CM影响脂质肉瘤细胞的生长和迁移.
在BMSC中,YAP1在促进脂肉瘤进展方面发挥着至关重要的作用.
在BMSC中准YAP1可能为脂肪肉瘤提供一种新的治疗方法.

关键词:

在 YAP1 沉默器中使用 YAP1 沉默器条件化介质是指一个条件化介质.人类的BMSCC 脂肪肉瘤 (liposarcoma) 是一种移民移民迁移迁移它们的扩散和扩散.

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