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收缩说明:MicroRNA-199a通过准SFRP5来调节大鼠心肌纤维化

M-H Chen¹, J-C Liu, Y Liu

¹Department of Emergency Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, China.

European review for medical and pharmacological sciences

|April 19, 2024

概括

由于对图像重复使用的担忧,对小鼠的microRNA-199a和心肌纤维化进行的这项研究已被撤回. 作者在PubPeer上提出关于图形重复的问题后,撤回了手稿.

科学领域:

心血管研究研究心血管研究
分子生物学分子生物学
药理学药理学是指药理学的学科.

背景情况:

心肌纤维化是一个重要的病理过程,有助于心力衰竭.
微RNAs (miRNAs) 正在成为心脏病的关键调节者.
分泌的纹相关蛋白5 (SFRP5) 已涉及纤维化的途径.

研究的目的:

在大鼠模型中研究微RNA-199a (miR-199a) 在调节心肌纤维化中的作用.
探索SFRP5是否是心脏纤维化背景下miR-199a的直接目标.

主要方法:

采用了心肌纤维化病的老鼠模型.
采用分子生物学技术来评估miR-199a的表达及其对心脏组织的影响.
通过生物信息学和实验验证,研究了miR-199a和SFRP5之间的相互作用.

主要成果:

这项研究旨在证明miR-199a在调节心肌纤维化方面发挥作用.
这些发现的目的是表明SFRP5是miR-199a的目标,介导其纤维化作用.
人们对数据完整性提出了担忧,特别是图2C和图4C中的数字重复.

结论:

作者最初认为,miR-199a通过向大鼠的SFRP5来调节心肌纤维化.
然而,由于对图像重复使用和数据完整性的重大担忧,该手稿已被撤回.
撤回强调了严格数据验证在科学出版中的重要性.

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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...