辛娜通液通过调节MDM2/STEAP3的作用来保护H9c2细胞免受H/R引起的损伤
Jiankun Cui1, Qinwen Wang2, Minghao Li3
1Department of Cardiology, The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, 150040, Heilongjiang, P. R. China.
PloS one
|April 19, 2024
概括
辛娜通格卢液通过增加小鼠双分钟2 (MDM2) 表达来保护心脏细胞免受损伤. 这种机制涉及调节MDM2/STEAP3,为心肌梗塞提供潜在的治疗基础.
科学领域:
- 心血管研究研究心血管研究
- 分子生物学分子生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 心肌梗塞 (MI) 造成了严重的健康负担.
- 临床上使用Xinnaotongluo液体治疗心脏病发作,但其机制尚不清楚.
- 了解分子通路对于有效治疗心脏病发作至关重要.
研究的目的:
- 阐明Xinnaotongluo液体在保护心肌细胞中的分子机制.
- 调查小鼠双分钟2 (MDM2) 在Xinnaotongluo液体影响中的作用.
- 为了探索MDM2和前列腺3 (STEAP3) 的六跨膜上皮抗原之间的相互作用在低氧/低氧化损伤.
主要方法:
- 使用暴露于低氧/低氧化 (H/R) 的H9c2细胞来模拟体外MI.
- 使用细胞计数套件-8.8评估细胞活力.
- 通过与酶相关的免疫吸收剂测定和西布洛特测量蛋白质表达.
主要成果:
- 在H9c2细胞中,H/R诱导了显著的损伤,并减少了MDM2表达.
- 通过Xinnaotongluo液体处理增加了MDM2的表达,减轻了H/R引起的损害.
- 过度表达MDM2增强了对氧化应激,炎症,亡和铁亡的保护作用.
- MDM2调节了p53和STEAP3的表达;STEAP3的过度表达抵消了MDM2的好处.
结论:
- 辛娜通流体通过上调MDM2.2,保护H9c2细胞免受H/R损伤.
- MDM2/STEAP3通路与Xinnaotongluo液体的心脏保护作用有关.
- 这项研究为Xinnaotongluo液体在MI中的治疗应用提供了理论基础.
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