来自Tenebrio molitor的寡的生产,表征和生物特性
Teresa Gonzalez-de la Rosa1, Sergio Montserrat-de la Paz1, Fernando Rivero-Pino1
1Department of Medical Biochemistry, Molecular Biology, and Immunology, School of Medicine, University of Seville, 41009 Seville, Spain; Instituto de Biomedicina de Sevilla, IBiS, Hospital Universitario Virgen del Rocio/CSIC/University of Seville, 41013 Seville, Spain.
Food chemistry
|April 19, 2024
概括
像Tenebrio molitor这样的食用昆虫提供了可持续的营养. 它们的蛋白质水解物具有显著的抗氧化和抗炎性质,具有功能性食品开发的潜力.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
- 营养科学 营养科学
背景情况:
- 可食用的昆虫,如Tenebrio molitor (食虫),是可持续的蛋白质来源.
- 来自Tenebrio molitor的蛋白质水解剂可以具有生物活性特性.
- 这些水解剂及其生物活性的表征对于食品应用至关重要.
研究的目的:
- 使用食品级蛋白质酶从Tenebrio molitor产生和表征蛋白质水解剂.
- 评估这些水解剂的体外抗氧化和抗炎活性.
- 为了确定体,并识别潜在的健康益处的生物活性.
主要方法:
- 使用Alcalase和Flavourzyme进行Tenebrio molitor蛋白的酶性水解.
- 使用INFOGEST协议进行体外消化.
- 对抗氧化活性进行DPPH激素清除试验.
- 对抗炎症作用的基因表达分析.
- 펩티돔的确定和在的分析.
主要成果:
- 布里奥芽粉和水解剂显示出强烈的DPPH激素清除活性 (EC50:0.300.87 mg/mL).
- 用组合蛋白酶产生的水解剂在消化后降低了亲炎性基因表达.
- 首次阐明了水解和消化物的完整层.
- 确定了40种生物活性,并选择用于in silico评估.
结论:
- 布里奥·莫莱托尔蛋白质的水解物具有显著的抗氧化和抗炎功能.
- 鉴定到的生物活性值得进一步研究功能性食品的开发.
- 可食用昆虫代表了人类消费的可行,营养和可持续的食物来源.
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