细胞外囊泡通过功能化的空心纤维膜进行选择性捕获
Simona Salerno1, Antonella Piscioneri1, Sabrina Morelli1
1Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, via P. Bucci, cubo 17/C, I-87036 Rende (CS), Italy.
Journal of colloid and interface science
|April 19, 2024
概括
研究人员开发了一种功能化的空心纤维膜,以隔离细胞外囊泡 (EVs). 这种方法有效地从生物流体中净化EV,有助于其诊断和治疗应用.
科学领域:
- 生物技术是生物技术.
- 膜科学 膜科学 膜科学
- 纳米技术 纳米技术
背景情况:
- 细胞外囊泡 (EVs) 在疾病病理学中至关重要,但孤立特定的EV群体仍然很困难.
- 目前的方法难以将纯EV与复杂的生物流体分开.
研究的目的:
- 开发一种酸功能化的空心纤维 (HF) 膜模块,以有效分离和丰富细胞外囊泡 (EVs).
- 为了优化EV隔离,使用与功能化膜集成的触流过 (TFF) 过程.
主要方法:
- 聚硫 (PSf) HF膜的功能化用 (BPt) 结合曲的纳米纤维.
- 修改HF膜以共聚化聚合物涂层,以减少非特异性结合,并通过点击化学实现联.
- 将BPt功能化的HF模块集成到用于EV隔离的TFF系统中.
主要成果:
- 该系统选择性地捕获EVs (<200nm),同时去除像专辑蛋白 (93%的去除) 这样的污染物.
- 孤立的EV表达了共同的标记物 (CD63,CD81,CD9,syntenin-1) 并保持了结构完整性.
- 该过程证明了低切割应力,高纯度和EV隔离中的可重复性.
结论:
- 与TFF集成的质功能化HF膜为高纯度的EV隔离提供了强大的方法.
- 这种方法促进了用于诊断和治疗应用的EVs的缩和净化.
- 该方法有效地去除污染物,保持电动汽车的完整性和功能.
相关概念视频
Pinocytosis
Cells use energy-requiring bulk transport mechanisms to transfer large particles, or large amounts of small particles, into or out of the cell. The cells envelop the particles in spherical membranes called vesicles or vacuoles. Vesicles that transport material into the cell are built from the cell membrane. These vesicles encapsulate external molecules and transport them into the cell in a process called endocytosis.
Pinocytosis
Cells use energy-requiring bulk transport mechanisms to transfer large particles or large numbers of small particles into or out of the cell. The cells envelop the particles in spherical membranes called vesicles or vacuoles. Vesicles that transport material into the cell are built from the cell membrane. These vesicles encapsulate external molecules and transport them into the cell in a process called endocytosis.
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The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
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