泰国菌 (Burkholderia thailandensis) 使用VI型分泌系统,在不触发宿主细胞反应的情况下溶解突起
Miro Thorsten Wilhelm Plum1, Hoi Ching Cheung1, Patricia Reist Iscar1
1Biozentrum, University of Basel, Spitalstrasse 41, 4056 Basel, Switzerland.
Cell host & microbe
|April 19, 2024
概括
泰国虫使用其VI型分泌系统-5 (T6SS-5) 来溶解宿主细胞突起,以便直接从细胞传播到细胞传播. 这种机制避免触发细胞防御反应,如自.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 细菌病原体的产生
背景情况:
- 像Burkholderia这样的细胞内细菌通过形成actin尾巴和突出进入相邻的宿主细胞来传播.
- 众所周知,VI型分泌系统-5 (T6SS-5) 中介细胞融合以促进细菌传播.
研究的目的:
- 为了研究T6SS-5在Burkholderia thailandensis中发挥的作用,Burkholderia thailandensis在细胞融合之外传播.
- 阐明B. thailandensis通过使用T6SS-5实现细胞间传播的机制.
主要方法:
- 活细胞成像观察细菌的传播和宿主细胞的反应.
- 在突起形成过程中分析dynamin-2招募和T6SS-5活动.
- 检测检测细胞融合,加勒-3,LC3和LAMP1的积累.
- 基因失活T6SS-5以评估其在细菌传播和宿主细胞反应中的作用.
主要成果:
- B. thailandensis 使用 T6SS-5 溶解膜突起,促进直接细胞间传播.
- 动氨酸-2的招募先于T6SS-5活动,导致动氨酸聚合和突起膜破坏.
- 突起溶解事件在很大程度上依赖于动氨酸,没有诱导细胞-细胞融合,并且逃避了加勒-3的检测.
- T6SS-5的无活化减少了突起溶解,但增加了宿主细胞中 galectin-3,LC3 和 LAMP1 的积累,表明细胞反应加剧.
结论:
- B. thailandensis在膜突起中特别激活T6SS-5,以破坏宿主细胞膜,以有效传播.
- 这种T6SS-5介导的溶解允许细菌在不激活细胞防御机制 (如自) 的情况下扩散.
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