一个小鼠模型的转录组和蛋白组特征是子烯诱导的精子DNA损伤
Chenming Zhang1, Yunfeng Ma2, Wenbang Liu2
1Henan University of Chinese Medicine, Zhengzhou, Henan 450046, China; The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, Henan 450003, China.
Reproductive toxicology (Elmsford, N.Y.)
|April 19, 2024
概括
甲 (BaP) 暴露显著增加了小鼠的精子DNA损伤. 这种损伤会影响关键的细胞通路,包括那些参与癌症和新陈代谢的细胞通路.
科学领域:
- 生殖毒理学 生殖毒理学
- 环境健康 环境健康
- 分子生物学分子生物学
背景情况:
- 甲 (BaP) 是一种已知的环境毒素.
- 精子DNA损伤是影响男性生育能力和后代健康的关键因素.
- 了解BaP诱导的精子DNA损伤的分子机制至关重要.
研究的目的:
- 为了复制一个小鼠模型的BaP诱导的精子DNA损伤.
- 为了研究BaP暴露后丸中转录组和蛋白组的变化.
- 为了确定受BaP诱导的精子DNA损伤影响的分子通路.
主要方法:
- 雄性小鼠在60天内被暴露在100毫克/公斤/天的子烯 (BaP) 中.
- 精子DNA碎片化指数 (DFI) 使用精子染色体结构测定 (SCSA) 进行评估.
- 用RNA测序 (RNA-seq) 和数据独立获取 (DIA) 质谱来进行转录基因和蛋白质基因分析.
主要成果:
- 暴露于BaP显著增加了精子DNA碎片化指数 (DFI).
- 与对照组相比,在暴露于BaP的组中观察到240个基因 (DEGs) 和616个蛋白质 (DEPs) 的差异表达.
- 丰富的途径包括与癌症相关的途径,PI3K-Akt信号传递,新陈代谢和MAPK信号传递.
结论:
- 甲 (BaP) 在小鼠模型中明显损害精子DNA.
- 暴露于BaP会在丸中诱导显著的转录和蛋白质变化.
- 受影响的分子通路包括癌症信号,PI3K-Akt,新陈代谢和MAPK信号,突出了潜在的毒性机制.
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