三种类型的酶完成了Angelica sinensis中的furanocoumarins核心骨架生物合成
Kaixuan Wang1, Huihui Zeng1, Yiqun Dai2
1Department of Resources Science of Traditional Chinese Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 210009, China.
这项研究确定了Angelica sinensis中的关键酶,这些酶完成了子 (FC) 生物合成. 了解这些植物代谢物为我们提供了关于它们多样化的起源和药理潜力的新见解.
科学领域:
- 植物生物化学 植物生物化学
- 代谢学 代谢学 代谢学
- 自然产品的生物合成.
背景情况:
- 甲 (FCs) 是重要的植物二次代谢物,具有多种药理活动.
- 安吉利卡辛尼斯 (Angelica sinensis) 是一个属类植物,富含FCs,但其生物合成途径尚不清楚.
- 阐明FC生物合成对于理解植物生理学和药用应用至关重要.
研究的目的:
- 为了阐明香 (FC) 生物合成的分子机制,在Angelica sinensis.
- 识别和描述负责FC核心骨架形成的酶.
- 提供有关FCs多元起源的见解.
主要方法:
- 基因组分析和关键酶的体外功能验证.
- 克隆和表征p-coumaroyl CoA 2'-基酶 (AsC2'H),UbiA 前基转移酶 (AsPT1,AsPT2) 和CYP736亚家族循环酶 (AsDC,AsOD).
- 酶活性测试以确定基质特异性和催化功能.
主要成果:
- 确定并验证了五种关键酶,这些酶在A. sinensis中完成了FC核心骨生物合成.
- AsC2'H催化贝利费龙的形成;AsPT1和AsPT2将贝利费龙转化为DMS和奥斯醇.
- AsOD表现出双功能循环酶活性,首选作用于奥斯醇.
结论:
- 描述的酶阐明了A. sinensis.中的FC生物合成的分子机制.
- 这项研究为研究FCs起源提供了新的见解和技术.
- 这些发现有助于了解植物的二次新陈代谢和潜在的制药应用.
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