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在低血糖期间,细胞使用葡萄糖-6-酸酶系统释放葡萄糖,以控制下丘脑能量平衡

María José Barahona¹, Luciano Ferrada², Matías Vera³

¹Laboratory of Neurobiology and Stem Cells, NeuroCellT, Department of Cellular Biology, Faculty of Biological Sciences, University of Concepcion, Concepcion, Chile; Center for Advanced Microscopy CMA BIO BIO, University of Concepcion, Concepcion, Chile; Laboratory of Appetite Physiology (FIDELA), Faculty of Medicine and Sciences, University San Sebastián, Concepción Campus, Concepción, Chile.

Molecular metabolism

|April 19, 2024

概括

脑下垂体细胞中的葡萄糖-6-酸酶 (G6Pase) 系统调节能量平衡. 击败tanycytes中的G6Pase会降低体重,脂肪和食物摄入量,影响新陈代谢.

科学领域:

神经内分泌学神经内分泌学
代谢调节代谢调节代谢调节
细胞生物学细胞生物学

背景情况:

肝脏利用内细胞网膜 (ER) 中的葡萄糖-6-酸酶 (G6Pase) 系统释放血糖.
G6Pase在下丘脑中的作用,特别是在tanycytes中,仍然在很大程度上未被探索.

研究的目的:

为了研究G6Pase系统在下丘脑前列腺细胞中的存在和功能.
确定tanycyte G6Pase在调节能量平衡和新陈代谢中的作用.

主要方法:

定量实时PCR (qRT-PCR) 和免疫组织化学评估G6Pase系统表达.
4D活细胞显微镜用于光葡萄糖模拟物 (2-NBDG) 的吸收.
在体内通过AAV1-shG6PT-mCherry注射在tanycytes中的G6pt的敲除.
评估体重,脂肪组织,食物摄入量,葡萄糖代谢和基因表达.

主要成果:

细胞表达G6Pase系统,将葡萄糖-6酸盐 (G6P) 隔离到ER中,并在低血糖期间通过GLUT释放葡萄糖.
在活体中,tanycytes中的G6pt被淘汰导致体重减少,白色脂肪组织 (WAT) 质量减少,并降低脂质生成基因的调节.
在tanycytes中选择性G6pt删除降低了食物摄入量,弧形核 (ARC) c-Fos表达,并在禁食小鼠中减少Npy mRNA.

结论:

与细胞相关的G6Pase系统是新陈代谢和能量平衡的关键调节者.
这一发现突显了tanycytes在中央代谢控制中的新角色.

关键词:

能源平衡能源平衡能源平衡 G6阶段系统的G6阶段.低血糖症是什么意思低血糖症细胞 (tanycytes) 是一种细胞.

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