在二甲基甘油酸转移酶的表达模式和调节中的差异导致鱼类中明显的肝脏甘油三沉积
Xiaojun Xiang1, Renlei Ji1, Shangzhe Han1
1Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs) & Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong, 266003, P.R. China.
Communications biology
|April 19, 2024
概括
由于DGAT酶的表达率更高,大黄鱼比彩虹鱼积累的肝脏甘油三化物更多. 这些在DGAT监管中的差异为研究肝脂沉积的鱼类模型提供了新的见解.
科学领域:
- * 鱼类脂质代谢的比较生理学和分子生物学.
- * 研究水生脊椎动物中甘油三沉积的遗传基础.
背景情况:
- *肝脏甘油三 (TAG) 积累与代谢障碍有关.
- *鱼类种类之间存在肝脏TAG积累的显著差异.
- *二甲基甘油酸转移酶 (DGAT1和DGAT2) 是TAG合成中的关键酶.
研究的目的:
- * 为了比较大黄鱼和彩虹鱼之间的肝脏TAG水平和DGAT基因表达.
- * 在体外研究DGAT1和DGAT2在TAG合成和脂质滴滴形成中的作用.
- * 在两种物种中,通过转录因子,特别是CREBH,探索DGAT1的转录调节.
主要方法:
- * 肝脏TAG水平和DGAT基因表达在同一饮食下对鱼和鱼的比较分析.
- *使用肝细胞进行体外研究,以评估DGAT1和DGAT2在TAG合成和脂质液滴形成中的功能.
- *研究油酸处理对DGAT表达的作用,并通过各种转录因子分析DGAT1促进体活性.
主要成果:
- * 大黄鱼的肝TAG水平明显高于彩虹鱼.
- *与鱼相比,鱼肝脏的DGAT基因表达明显高于鱼.
- *DGAT1和DGAT2对于TAG合成和两种物种肝细胞中的脂质液滴形成至关重要.
- * 油酸处理提高了鱼但不是鱼肝细胞中的DGAT1表达.
- *DGAT1被确定为鱼中的CREBH标基因,但不是鱼.
结论:
- *肝脏DGAT表达和转录调节的特定物种差异有助于在鱼和鱼之间形成不同的肝脏TAG沉积模式.
- *这些发现凸显了使用大黄鱼和彩虹鱼作为研究肝脏TAG代谢的独特鱼类模型的潜力.
- * 了解这些差异为鱼类脂质代谢背后的分子机制提供了新的视角.
相关概念视频
Cell Specific Gene Expression
13.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.6K
Fats as Energy Storage Molecules
25.1K
Triglycerides are a form of long-term energy storage molecules. They are made of glycerol and three fatty acids. To obtain energy from fat, triglycerides must first be broken down by hydrolysis into their two principal components, fatty acids and glycerol. This process, called lipolysis, takes place in the cytoplasm. The resulting fatty acids are oxidized by β-oxidation into acetyl-CoA, which is used by the Krebs cycle. The glycerol that is released from triglycerides after lipolysis...
25.1K
Overview of Fatty Acid Metabolism
30.6K
Lipids also are sources of energy that power cellular processes. Like carbohydrates, lipids are composed of carbon, hydrogen, and oxygen, but these atoms are arranged differently. Most lipids are nonpolar and hydrophobic. Major types include fats and oils, waxes, phospholipids, and steroids.
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
30.6K
Cholesterol: Significance and Regulation
540
Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
Considering cholesterol and...
540
Blood Studies for Cardiovascular System III: Serum Lipid Profile
156
Understanding serum lipids is crucial for maintaining cardiovascular health and preventing heart disease and stroke.
Serum lipids are fats and fatty substances in the blood and are crucial for various bodily functions, including energy storage, cellular structure, and hormone production. Serum lipids consist of cholesterol, triglycerides, and phospholipids.
Cholesterol is a soft, fat-like substance found in all body cells. It is crucial for producing hormones, vitamin D, and substances that aid...
Serum lipids are fats and fatty substances in the blood and are crucial for various bodily functions, including energy storage, cellular structure, and hormone production. Serum lipids consist of cholesterol, triglycerides, and phospholipids.
Cholesterol is a soft, fat-like substance found in all body cells. It is crucial for producing hormones, vitamin D, and substances that aid...
156
Overview of Lipid Metabolism
1.5K
Lipid metabolism is a crucial process in the human body that involves the synthesis and degradation of lipids. This process is essential for energy production, cell membrane formation, and hormone production, among other functions.
Lipolysis: The Breakdown of Lipids:
Lipolysis is the process of breaking down lipids, particularly triglycerides, into glycerol and fatty acids. This process typically occurs in the adipose tissue and is triggered by various hormones, including glucagon and...
Lipolysis: The Breakdown of Lipids:
Lipolysis is the process of breaking down lipids, particularly triglycerides, into glycerol and fatty acids. This process typically occurs in the adipose tissue and is triggered by various hormones, including glucagon and...
1.5K


