描述BACILUS AMYLOLIQUEFACIENS PM415作为一种潜在的生物保存益生菌的特征
Jingkang Ye1, Haiyang Wu2, Li Feng2
1School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, 510006, P. R. China.
Archives of microbiology
|April 20, 2024
概括
一种新的Bacillus菌株Bacillus amyloliquefaciens PM415有效地抑制了动物料中的真菌生长. 这种安全,非致病性益生菌为生物保存提供了有希望的解决方案,提高了料的安全性和质量.
科学领域:
- 微生物学 微生物学
- 动物科学动物科学
- 食品科学 食品科学 食品科学
背景情况:
- 动物料容易受到真菌污染,需要有效的生物保存策略.
- 虽然Lactobacillus和Bifidobacteria是众所周知的益生菌,但具有抗真菌性质的Bacillus物种被探索的较少.
- 需要新的抗真菌能力的细菌菌株来提高动物料的安全性.
研究的目的:
- 查具有抗真菌活性的新型细菌菌株.
- 为了识别和描述一个有前途的细菌菌株,用于动物料的生物保存.
- 调查所选菌株抗真菌特性背后的机制.
主要方法:
- 选56种细菌菌株的循环脂基因.
- 使用Aspergillus niger的抗真菌测试,包括对抗测试和扫描电子显微镜 (SEM).
- 全基因组测序,遗传学分析,in silico安全性评估和RNA测序 (RNA-seq).
主要成果:
- 巴西氨基面菌PM415表现出优异的抗真菌活性对黑色阿斯伯菌.
- 在治疗过程中,SEM揭示了Aspergillus niger hyphae的显著形态损伤.
- 基因组分析证实了其非致病性质,并确定了潜在的抗真菌基因集群.
- RNA-seq表明了对真菌对抗反应的代谢转变,包括运输和非核糖体生物合成的变化.
结论:
- 乙氨基面菌PM415是一种安全有效的益生菌候选物,用于防止动物料中的真菌生长.
- 该菌株具有强烈的抗真菌特性,可能由循环脂介导.
- 这项研究扩大了用于农业应用的细菌益生菌的范围.
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