Circ_0087851通过触发miR-593-3p/BAP1介导的铁死来抑制结直肠癌的恶性进展
Ming Huang1, Ting Gao1, Xianyong Chen1
1Department of Pathology, Affiliated Hospital of Xiangnan University, Chenzhou, 423000, Hunan, China.
Journal of cancer research and clinical oncology
|April 20, 2024
概括
循环RNAs (circRNAs) 调节结直肠癌 (CRC) 的进展. 这项研究发现circ_0087851通过miR-593-3p/BAP1通路促进铁亡,从而抑制CRC,从而成为潜在的治疗标.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 循环RNAs (circRNAs) 是瘤发生的关键调节者,包括结直肠癌 (CRC).
- 铁亡,一种独特的细胞死亡形式,在瘤恶性瘤中起作用.
- 了解CRC和铁亡中的circRNA功能对于治疗开发至关重要.
研究的目的:
- 为了研究circ_0087851在结直肠癌进展中的作用.
- 探索circ_0087851对CRC中铁亡的影响.
- 阐明circ_0087851在CRC中的功能背后的分子机制.
主要方法:
- 定量实时PCR (qRT-PCR) 用于评估circ_0087851的表达.
- 在体外测试 (CCK-8,殖民地形成,Transwell) 和体内异种移植模型来评估CRC细胞行为和瘤生长.
- 生物化学分析测量铁灭标记物 (铁,Fe2+,脂质ROS).
- 生物信息学, luciferase 记者和RNA 拉下测试以确定分子相互作用.
主要成果:
- 在CRC组织和细胞中,Circ_0087851的表达显著减少.
- 过度表达circ_0087851抑制了CRC细胞的增殖,迁移,入侵,并诱导了ferroptosis在体外.
- 在体内抑制了circ_0087851的瘤生长.
- Circ_0087851充当了miR-593-3p的分子海绵,它准了BAP1,揭示了circ_0087851/miR-593-3p/BAP1轴.
结论:
- 在CRC中,Circ_0087851作为瘤抑制剂和铁亡促进剂起作用.
- 在miR-593-3p/BAP1轴中介于circ_0087851.1.的瘤抑制作用.
- Circ_0087851是一个有前途的生物标志物和结直肠癌管理的治疗标.
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