温暖后到胚胎移植时间对人体胚胎细胞代谢和怀孕结果的影响
Goli Ardestani1, Marion Martins2,3, Olcay Ocali2
1Boston IVF - IVIRMA Global Research Alliance, Waltham, MA, 02451, USA. gardestani@bostonivf.com.
Journal of assisted reproduction and genetics
|April 20, 2024
概括
对于冷胚胎移植的加热后培养时间不会影响怀孕结果,如活产或流产率. 这一发现支持在生育治疗中灵活的临床安排.
科学领域:
- 生殖科学 生殖科学
- 胚胎学 胚胎学
- 代谢成像 - 代谢成像
背景情况:
- 优化体外受精 (IVF) 协议对于提高妊娠成功率至关重要.
- 了解实验室程序的影响,如温暖后的培养时间,对胚胎发育和生存能力至关重要.
研究的目的:
- 为了研究变化后温暖培养持续时间对胚胎细胞代谢的影响.
- 确定保温后培养时间是否会影响冷胚胎移植 (FET) 后的怀孕和活产率.
主要方法:
- 一个回顾性队列研究分析了11520个单个FET周期 (2015年1月至2020年12月).
- 胚胎根据加热后的培养时间 (0-8小时) 分类.
- 非侵入性代谢成像 (光终身成像显微镜 - FLIM) 评估了培养期间的胚胎细胞代谢.
主要成果:
- 在不同的后变暖文化时期,没有观察到活产率 (38-52%) 或流产率 (5-11%) 的统计学上显著差异.
- 怀孕结果无论培养时间如何,都保持一致,即使通过植入前遗传测试 (PGT-A) 用来分层.
- 代谢分析显示,在温后长达10小时培养的胚芽细胞中发生的变化很小.
结论:
- 温暖后培养时间的变化不会显著影响冷胚胎移植中的流产或活产率.
- 这些发现为临床实践提供了灵活性,特别是那些员工数量有限或患者数量高的临床实践.
- 该研究支持调整FET调度的安全性和有效性,而不会影响生殖结果.
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