从患有蜂炎的肉中回收的大肠杆菌分离物的分子特征
Anja Müller1, Kathrin Schulze Bernd2, Diana Seinige3
1Institute for Veterinary Food Science, Justus Liebig University Giessen, Giessen, Germany.
Poultry science
|April 20, 2024
概括
导致 brojler 蜂炎的禽病原体 Escherichia coli (APEC) 显示出遗传多样性和高抗菌耐药性. 虽然对关键抗生素的耐药性很低,但在德国家禽养殖场中普遍存在对安培素等常见药物的广泛耐药性.
科学领域:
- 兽医微生物学 兽医微生物学
- 抗微生物耐药性 抗微生物耐药性
- 禽类的健康 禽类的健康
背景情况:
- 由于禽病原菌大肠杆菌 (APEC) 引起的 brojlers中的鸟类细胞炎,导致了由于尸体排斥而造成的重大经济损失.
- 了解APEC分离品的遗传关系和抗菌素耐药性概况对于有效的禽类疾病管理和控制至关重要.
研究的目的:
- 分析E.大肠杆菌与细胞炎的肉分离的遗传关系和抗菌素耐药性.
- 确定是否存在APEC的克隆传播或临床分离物体是否表现出遗传多样性.
主要方法:
- 在77个德国家禽养殖场中,从77只患病的肉中分离和鉴定了107种大肠杆菌菌株.
- 类型化方法包括血型定型,基于PCR的类型定型,以及用脉冲场凝电泳 (PFGE) 进行宏限制分析.
- 对毒性基因和抗菌素易感性 (磁盘扩散) 的研究,然后对抗性基因进行PCR.
主要成果:
- 显著比例的分离物属于主要的APEC血清型O78:K80;基因组D是最常见的.
- 宏观限制分析揭示了一些聚类的异质结果,表明分离物之间的多样性和潜在相关性.
- 观察到高抗微生物耐药性:83.8%对至少一种药物耐药,40%对至少三种药物耐药. 抗西林耐药性 (blaTEM) 是最常见的.
- 对于西普罗夫洛克萨 (8%) 发现了较低的抗药率,对第三代类素或卡巴类药物没有抗药性.
结论:
- 与鸟类细胞炎相关的基因多样化的APEC菌株存在于德国家禽养殖场内和其中.
- 广泛的抗微生物药物耐药性是令人担忧的,但对关键重要的抗微生物药物的耐药性在这些临床隔离物中仍然很低.
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