利用人工智能驱动的方法,从新分离的Streptomyces rutgersensis AW0808中获得增强的英多尔-3-酸
Wiem Alloun1, Mohammed Berkani2, Amin Shavandi3
1Laboratory of Mycology, Biotechnology and Microbial Activity, Department of Applied Biology, BP, 325 Aïn El Bey Road, Constantine 25017, Algeria; The BioMatter Lab, École Polytechnique de Bruxelles, Université Libre de Bruxelles (ULB), Avenue F.D. Roosevelt, 50-CP 165/61, 1050 Brussels, Belgium; Department of Life, Health and Environmental Sciences, University of L'Aquila, Coppito, 67100 L'Aquila, Italy.
Environmental research
|April 20, 2024
概括
研究人员确定了一种新型的Streptomyces rutgersensis菌株,AW08,用于高醇-3-酸 (IAA) 生产. 人工智能利用消耗的咖啡粉优化了IAA生物合成,实现了显著的产量增加.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 人工智能的人工智能
背景情况:
- 印度醇-3-酸 (IAA) 是一种重要的植物激素.
- 农业废物利用为生物工艺提供可持续的原料.
- 合成IAA生产带来了环境和成本问题.
研究的目的:
- 从阿尔及利亚的沉积物中分离并识别出一种高产的IAA产生细菌菌株.
- 为了优化IAA生物合成,使用消耗的咖啡粉作为基板.
- 应用人工智能来最大限度地提高IAA的产量.
主要方法:
- 隔离和选用于IAA生产的行为细菌菌株.
- 16S rRNA基因测序用于细菌识别.
- 响应表面方法 (RSM) 使用盒式设计 (BBD).
- 有限内存的布劳登-弗莱彻-戈德法尔布-沙诺盒 (L-BFGS-B) 优化算法.
主要成果:
- 一种新型菌株被鉴定为Streptomyces rutgersensis AW08,被分离出来.
- S. rutgersensis AW08产生了26.116 ± 0.61μg/mL的IAA. 这是一个很好的结果.
- 使用L-BFGS-B和BBD-RSM的AI驱动优化分别提高了IAA生产的5.43倍和4.2倍.
- 在最佳条件下,IAA的产量高达169.18微克/毫升.
结论:
- S. rutgersensis AW08 是一个强大的IAA生产者,首次在阿尔及利亚被发现.
- 基于AI的优化显著提高了IAA的生产效率.
- 消耗的咖啡用于可持续的IAA生产是可行的.
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