在成熟的久居马中,对重复的部肌肉微生物检查的局部和全身反应
Jessica L Artman1, Lauren T Wesolowski1, Pier L Semanchik1
1Department of Animal Science, Texas A&M University and AgriLife Research, 2471 TAMU, College Station, TX 77843, USA.
Journal of equine veterinary science
|April 20, 2024
概括
马的重复肌肉活检对线粒体功能和抗氧化剂状况的影响最小. 血清肌酸激酶 (CK) 活性暂时增加,这表明在马研究中重复采集组织时要谨慎.
科学领域:
- 马类生理学 马类生理学
- 运动生理学 运动生理学
- 肌肉生物学 肌肉生物学
背景情况:
- 在马研究中,重复的肌肉活检是常见的.
- 对线粒体功能,抗氧化剂状况和炎症的潜在影响尚未完全理解.
研究的目的:
- 为了研究重复的部中部肌肉集合对马体生理学的影响.
- 评估线粒体功能,抗氧化剂状态,炎症和肌肉损伤标记物的变化.
主要方法:
- 26匹马在不同的时间点 (0,6,12,24小时) 进行了多次肌肉活检.
- 血液样本每6小时收集一次,持续72小时.
- 分析了线粒体功能 (电子转移能力,LEAK,酸盐合成酶活性),血清肌酸激酶 (CK),全血超氧化物失调酶,血清皮质醇和细胞因子度.
主要成果:
- 线粒体功能标志物 (复合II,LEAK,CS活动) 在12小时内出现过渡性变化.
- 血清CK活性在采集后6小时增加,但仍在正常范围内.
- 反氧化剂状态和炎症标志物没有受到重复肌肉收集的显著影响.
结论:
- 重复的肌收集对马中的大多数生理标志物产生了有限的短期影响.
- 血清CK活性升高在重复组织采样的背景下需要谨慎解释.
- 这些发现支持在非压力马中重复肌肉活检的安全性,并谨慎解释CK水平.
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