内源性以太脂质通过调节SK3通道来差异地促进瘤的攻击性
Marion Papin1, Delphine Fontaine1, Caroline Goupille2
1Niche, Nutrition, Cancer & Oxidative metabolism (N2COx), UMR 1069, INSERM, University of Tours, Tours, France.
Journal of lipid research
|April 20, 2024
概括
内源性以太脂调节SK3通道,影响癌细胞的攻击性. 抑制以太脂质合成减少SK3表达和关键癌细胞功能,提供新的治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- SK3通道与促进瘤的攻击性有关.
- 内源性乙脂在调节SK3通道中的作用在很大程度上是未知的.
- 之前的研究强调了SK3通过合成乙脂的调节.
研究的目的:
- 调查内源性基和基乙烯脂在控制SK3通道中的作用.
- 阐明以太脂对癌细胞生物学的影响,包括迁移和入侵.
- 确定影响SK3表达和活性的特定乙烯脂质.
主要方法:
- 在以太脂质合成中的关键酶的基因沉默 (基甘油酸盐合成酶,等离子体乙醇胺脱酶1).
- 对微RNA (miR-499,miR-208) 表达水平的分析.
- 测量SK3依赖的进入,细胞迁移和矩阵金属蛋白酶9依赖的细胞粘附和侵入.
- 在临床样本中对基因和microRNA表达的相关性分析.
主要成果:
- 抑制乙脂合成酶降低了SK3的表达.
- 这种降低是由增加的miR-499和miR-208表达的介导.
- 减少SK3表达导致减少的进入,细胞迁移和入侵.
- 确定了促进SK3表达的特定脂,对SK3活性有不同的影响.
结论:
- 内源性以太脂质通过调节SK3离子通道来差异控制瘤的攻击性.
- 这项研究提供了通过离子通道调节对瘤侵略性以太脂质控制的第一个证据.
- 研究结果表明,针对以太脂质代谢的潜在治疗策略可以调节癌症中的离子通道功能.
相关概念视频
02:17The Tumor Microenvironment
6.6K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.6K
02:38Mitogens and the Cell Cycle
6.5K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.5K
01:22Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.2K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.2K
03:03mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
01:11IP3/DAG Signaling Pathway
12.0K
Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
12.0K
01:17Intracellular Signaling Affects Focal Adhesions
2.7K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
2.7K