对预测蛋白质稳定性变化对变化的审查
Yiling Qiu1,2, Tao Huang1, Yu-Dong Cai3
1Bio-Med Big Data Center, CAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China.
Proteomics
|April 21, 2024
概括
预测遗传变异如何影响蛋白质稳定性对医学和工业至关重要. 本综述涵盖了用于蛋白质稳定性预测的计算方法,数据库和实验技术.
科学领域:
- 生物化学和分子生物学
- 计算生物学 计算生物学
- 生物信息学是一种生物信息学.
背景情况:
- 蛋白质稳定性对于生物功能和生物技术应用至关重要.
- 了解突变对蛋白质稳定性的影响对于疾病研究和蛋白质工程至关重要.
- 稳定性变化的准确预测有助于合理的蛋白质设计.
研究的目的:
- 审查目前用于预测因突变而导致的蛋白质稳定性变化的计算方法.
- 调查包含蛋白质突变数据和热力学参数的数据库.
- 讨论高通量评估蛋白质稳定性的实验技术.
主要方法:
- 审查用于蛋白质稳定性预测的最先进的计算方法.
- 分析各种计算模型的特征,算法和预测性能.
- 对计算预测的实验验证方法的描述.
主要成果:
- 识别和分类众多公开可用的数据库,用于蛋白质稳定性预测.
- 详细概述计算方法,包括它们的基础算法和预测准确性.
- 适用于高通量稳定性评估的实验技术摘要.
结论:
- 在计算蛋白质稳定性预测方面取得了重大进展.
- 在提高预测准确性和整合各种数据类型方面仍然存在挑战.
- 未来的研究可能将重点放在先进的建模技术和实验计算集成上.
更多相关视频
08:13Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
Published on: March 4, 2017
39.0K
07:22How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
Published on: February 11, 2019
28.2K
相关概念视频
01:53RNA Stability
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
02:26Conservation of Protein Domains Over Different Proteins
Protein domains are small structurally independent units that are part of a single amino acid chain. Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...
01:53RNA Stability
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
01:26Protein Modifications in the RER
Modification of secretory and transmembrane proteins entering the rough ER begins in the ER lumen. These modifications aid in protein folding and stabilize the acquired tertiary structure. Protein modifications in the rough ER co-occur at different stages of protein folding.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal sequences.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal sequences.
01:29Protein Folding Quality Check in the RER
ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
01:26Bacterial Protein Maturation
Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...