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从三种微藻中提取多糖的提取,结构特征和抗氧化活性
Yang Zhao1, Chun Han1, Yangyingdong Wu1
1Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China.
The Science of the total environment
|April 21, 2024
概括
来自Golenkinia sp.,Chlorella sorokiniana和Spirulina subsalsa的微藻类多糖体具有抗氧化特性. 螺旋藻亚盐多糖 (SPS) 显示出最高的产量和活性,通过Nrf2-ARE通路调节氧化应激.
科学领域:
- 生物化学 生物化学
- 海洋生物学 海洋生物学
- 生物技术是生物技术.
背景情况:
- 微藻多糖因其在控制氧化损伤方面的潜力而得到认可.
- 氧化应激与各种生理和病理条件有关.
研究的目的:
- 研究来自Golenkinia sp.的多糖的产量,结构和抗氧化作用. (GPS),克洛雷拉索罗基尼亚纳 (CPS) 和螺旋藻亚盐 (SPS).
- 阐明这些微藻多糖类调节氧化应激的机制,重点关注基因表达途径.
主要方法:
- 使用标准化方法从三种微藻中提取多糖.
- 多糖结构的表征,包括分子量分布和单糖组成 (主要是银糖).
- 在体外抗氧化活性测定和基因表达分析 (Nrf2-ARE通路) 以评估氧化应激调节.
主要成果:
- 所有提取的多糖化合物 (GPS,CPS,SPS) 都是异多糖化合物,其中具有很高比例的小分子分数,主要是银河糖.
- SPS表现出最高的产量和抗氧化活性,这归因于其较高的小分子分数和银河糖的比例.
- GPS,CPS和SPS在体外显示出显著的抗氧化能力和有效调节细胞氧化应激,而SPS显示出略有优异的效果.
结论:
- 微藻多糖,特别是SPS,具有显著的抗氧化和氧化应激调节特性.
- Nrf2-ARE信号通路是这些多糖体对细胞氧化应激产生影响的关键机制.
- 这些发现支持微藻多糖的健康产品应用和进一步研究的潜力.
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