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通过喷雾干燥和冷化稳定传染性包裹病毒
Holly J Coleman1, Daniel K Schwartz1, Joel L Kaar1
1Department of Chemical and Biological Engineering, University of Colorado Boulder, CO 80303, USA.
Journal of pharmaceutical sciences
|April 21, 2024
概括
喷雾干燥和冷化在干燥矩阵中稳定包裹病毒,保持感染力并增强热稳定性,以改善基因和免疫疗法传递.
科学领域:
- 生物技术是生物技术.
- 病毒学 病毒学
- 材料科学 材料科学 材料科学
背景情况:
- 包裹病毒是有效的基因和免疫疗法传递载体.
- 液体配方中的病毒不稳定性限制了保质期和获取.
- 目前的储存要求增加了成本,并限制了患者的访问.
研究的目的:
- 开发稳定,干燥的包裹病毒配方.
- 评估喷雾干燥和冷化对病毒感染性和稳定性的影响.
- 为了提高包裹病毒的热稳定性,用于治疗应用.
主要方法:
- 用喷雾干燥和冷化技术封装了包裹病毒.
- 使用多糖矩阵嵌入病毒.
- 在各种热应激条件下测量病毒标位和感染力.
主要成果:
- 在喷雾干燥或冷化后没有观察到病毒标位的损失.
- 嵌入式病毒显示显著增强了热稳定性.
- 病毒在暴露于85°C1小时和30°C10周后仍然保持感染力.
结论:
- 喷雾干燥和冷化是创建稳定干燥病毒配方的有效方法.
- 这些干式配方显著提高了包裹病毒的热稳定性.
- 这种方法提供了一个有希望的策略,以克服病毒载体存储和基因和免疫疗法的可访问性方面的局限性.
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