增强了直接气态CO2的固定,从而产生更高的生物酸和选择性
Ziyi Yang1, Wanling Wu1, Qing Zhao1
1Biomass Energy and Environmental Engineering Research Center, Beijing University of Chemical Technology, Beijing 100029, China; College of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
Journal of environmental sciences (China)
|April 21, 2024
概括
这项研究优化了使用二氧化碳 (CO2) 和微生物催化剂的生物酸生产. 研究人员通过调整碳来源和使用逐步增加二氧化碳来提高二氧化碳固定和酸产量,从而改善碳捕获和循环利用.
科学领域:
- 生物技术是生物技术.
- 生物化学工程 生物化学工程
- 微生物催化剂的作用
背景情况:
- 用二氧化碳 (CO2) 来生产生物酸为碳捕获和循环 (CCR) 提供了一种可持续的方法.
- 艾克蒂诺巴西勒斯 (Actinobacillus succinogenes) 和巴斯菲亚 (Basfia succiniciproducens) 是这种生物过程的有希望的微生物催化剂.
研究的目的:
- 为了优化增强二氧化碳固定和生物糖酸生产的条件.
- 调查无机碳源组成和添加策略对微生物转化效率的影响.
主要方法:
- 使用MgCO3和CO2对无机碳和葡萄糖度的评估2.
- 调整碳酸盐与二氧化碳的比率,以最大限度地利用气态二氧化碳.
- 对参与葡萄糖代谢的关键酶的分析.
- 逐步实施二氧化碳添加战略.
主要成果:
- 最佳条件被确定为6g C/L MgCO3和24g C/L 葡萄糖,产量>30g/L酸和>40%的碳产量.
- 1:9的碳酸盐:CO2比率 (以碳质量为单位) 最大化了CO2固定 (~15毫克C/L·hr)) 并抑制了乳酸.
- 高的气态二氧化碳比率增强了烯酸酸碳酸酶与乳酸脱酶的比率,有利于酸的产生.
- 逐步添加的二氧化碳使无机碳利用率提高了50%-65%,酸的选择性增加了20%-30%.
结论:
- 通过仔细控制碳源成分和养策略,可以实现微生物对二氧化碳转化为生物酸的最佳转化.
- 增强的二氧化碳固定和酸生产与代谢途径的转变有关,有利于酸合成.
- 这种方法为碳捕获和回收提供了一种可行的方法,同时生产出一种有价值的平台化学物质.
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