人类母乳外体miRNA受过早分娩的影响,影响神经发育
Ling Ma1, Yanyan Huo1, Qingyuan Tang2
1Department of Child Health Care, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200062, China.
Molecular nutrition & food research
|April 21, 2024
概括
过早分娩会改变母乳外体的微RNA (miRNA) 概况,影响婴儿的神经发育. 特定的miRNAs,如miR-3168,对于神经干细胞分化至关重要.
科学领域:
- 生物化学 生物化学
- 发展生物学 发展生物学
- 遗传学 遗传学 是一个
背景情况:
- 人类母乳 (HBM) 含有外体微RNA (miRNAs),对婴儿发育至关重要.
- 早产可能会改变这些必不可少的HBM组件的组成.
研究的目的:
- 为了研究长期和早产HBM的外体miRNA配置文件.
- 确定关键的差异表达miRNA及其在婴儿神经发育中的作用.
主要方法:
- 从终期和前期的HBM中对外体miRNA的高通量测序.
- 生物信息分析以确定丰富和差异表达的miRNAs.
- 在体外研究以确认特定miRNAs在神经干细胞分化中的作用.
主要成果:
- Let-7和miR-148家族是HBM外基因组中最丰富的miRNA.
- 在早产的HBM外体中,有23个miRNA被上调,15个被下调.
- miR-3168,在早产HBM中高度上调,准SMAD和MAPK通路,这对神经发育和神经干细胞分化至关重要.
结论:
- 过早分娩显著影响HBM中的外体miRNA表达.
- 在早产HBM中改变的miRNA配置文件可能会对早产婴儿的神经发育产生负面影响.
- miR-3168是神经发育过程的关键调解者,受HBM外体的影响.
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