使用光,散射和吸收光谱和它们的分析应用,研究乙红和心脏药物阿米奥达龙之间的相互作用
Huda Salem AlSalem1, Sara Naif Alharbi1, Mona Saad Binkadem2
1Department of Chemistry, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.
概括
这项研究引入了使用红色素B检测阿米奥达龙的新光谱方法. 这些基于共振雷利散射和光的方法提供了药物配方中阿米奥达龙的敏感和可靠量化.
科学领域:
- 分析化学 分析化学
- 频谱学是一种光谱学.
- 药品分析 药品分析
背景情况:
- 阿米奥达龙是一种关键的抗心律失常药物,需要准确量化.
- 现有的分析方法可能缺乏灵敏度或需要复杂的程序.
- 红素B是一种光染料,具有作为光谱探测器的潜力.
研究的目的:
- 开发用于确定阿米奥达龙的新型光谱方法.
- 为了利用红素B作为一个探针来检测阿米奥达龙.
- 根据ICH指南验证开发的方法.
主要方法:
- 在酸性溶液中形成乙红和阿米奥达龙之间的关联复合物.
- 监测红素B的共振雷利散射 (RRS),吸收和光谱的变化.
- 优化用于光谱测量的分析条件.
- 开发的RRS,光度和度方法的验证.
主要成果:
- 红色素B-阿米奥达龙复合体的形成增强了RRS和吸收性,同时灭了光.
- 在特定范围内的光谱变化和阿米奥达龙度之间建立了线性关系.
- 达到的检测值的低极限:0.52 ng/mL (光谱),0.051 μg/mL (RRS) 和0.075 μg/mL (度).
- 这些方法在商用阿米奥达龙片中应用时显示出良好的恢复率.
结论:
- 开发了敏感和验证的光谱方法来定量阿米奥达龙.
- 红素B作为一种有效的探针用于阿米奥达龙的确定.
- 提出的方法适用于在药品中分析阿米奥达龙.
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