通过中间原始体,有序的规范和精确的层状部署皮质谷氨基基投射神经元类型通过中间原始体
bioRxiv : the preprint server for biology
|April 22, 2024
概括
在发育中的大脑皮层中,间接神经发生 (iNG) 会产生多样化的投射神经元 (PN). 这个过程偏离了传统的"内外"模型,揭示了更复杂的,特定于层次的神经元生成方案.
科学领域:
- 神经科学是一个神经科学.
- 发展生物学 发展生物学
- 遗传学 是一个遗传学.
背景情况:
- 大脑皮层从放射性质原始体 (RGs) 发展出谷氨基质投影神经元 (PNs).
- PNs通过直接的神经发生或间接的神经发生 (iNG) 通过中间原始体 (IPs) 生成.
- "内外"模型描述了PN向皮质层的迁移,但其意义和iNG的作用尚不清楚.
研究的目的:
- 研究间接神经发生 (iNG) 在投射神经元 (PNs) 层状部署中的作用.
- 为了确定PN生成是否遵循已建立的"内外"皮质形成模型.
- 阐明从中间祖先 (IP) 来生成PN的时间动态.
主要方法:
- 在小鼠中利用遗传策略将PN出生日期与投影映射联系起来.
- 采用了来自IP的PN生成的谱系追踪和时间分析.
- 分析了从IPs获得的PNs的层状分布.
主要成果:
- 来自IP的PN的层状部署显著偏离了"内外"规则.
- 用于非连续层的PNs是同时生成的.
- 同一个层内的不同PN类型是在非连续的时间生成的.
- 每个IP都受到命运的限制,产生一对几乎相同的PN.
结论:
- 皮质生成涉及由命运受限的IP时间队列对多个PN类型的顺序和并发规范.
- 产生和部署PN类型到特定的皮质层并不严格遵守"内外"的顺序.
- 这种复杂的iNG方案可以促进皮质输出电路的有组织组装.
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