在常规和微波加热下,在溶解和整洁系统中加强酶 sorbityl 酸盐生产
André Delavault1, Oleksandra Opochenska1, Sonja Schönrock1
1Technical Biology, Institute of Process Engineering in Life Sciences II, Karlsruhe Institute of Technology, Karlsruhe 76131, Germany.
ACS omega
|April 22, 2024
概括
这项研究通过优化温度和加热方法,通过使用Novozym 435增强了sorbityl laurate的生产. 微波加热在深度环氧溶剂 (DES) 中显著增加了时空产量,甚至使无溶剂反应成为可能.
科学领域:
- 生物催化和绿色化学
- 酶工程和工业生物技术
背景情况:
- 甘油脂,像糖酒精 Esters,有多种不同的应用.
- 使用有机或深溶剂 (DES) 的脂酶催化生物转化显示出工业的前景,但在生产时间和生产率方面面临挑战.
- 加强这些生物催化工艺对于工业采用至关重要.
研究的目的:
- 为了优化sorbityl laurate的生产,一个模型糖脂,使用Novozym 435.5.
- 研究反应温度,加热方法 (传统与微波) 和溶剂系统 (DES与2M2B) 对生物催化性能的影响.
- 为提高工业可扩展性而提高时空产量和整体产品产量.
主要方法:
- 使用固定脂酶Novozym 435.5进行sorbityl laurate的酶合成.
- 传统加热与微波加热的比较分析.
- 在2-甲基-2-butanol (2M2B) 和深溶解剂 (DES) 中反应性能的评估.
- 反应温度从50°C到90°C的系统变化.
主要成果:
- 将反应温度提高到90°C显著提高了2M2B和DES的时空产量和产品产量.
- 在2M2B中以90°C的常规加热方式,在90分钟内实现了98%的转换,产生118.0 ± 3.6 g/L·h.
- 微波加热在DES中显示,与传统加热相比,在50°C时的时空产量增加了7倍,在90°C时增加了16倍.
- 微波辐射促进了无溶剂合成,达到高达13.3 ± 2.3 g/L·h的生产率.
结论:
- 更高的反应温度 (高达90°C) 有助于加强酸生产.
- 微波加热比传统加热提供了显著的优势,特别是在DES中,并且可以实现无溶剂生物催化.
- 优化条件,特别是使用微波辐射,可以显著提高工业应用的脂酶催化甘油脂合成的生产率和效率.
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