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Updated: Jul 7, 2026

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
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PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase

Published on: September 26, 2020

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来自微重力培养的晶体的中子衍射揭示了托合成酶的内部阿尔迪米因形式的活性位点

Victoria N Drago1,2, Juliette M Devos3,4, Matthew P Blakeley5

  • 1Department of Chemistry and Biochemistry, University of Toledo, Toledo, OH 43606, USA.

Cell reports. Physical science
|April 22, 2024
PubMed
概括

皮里多克萨尔5'-酸盐 (PLP),维生素B6的活性形式,对于生物合成至关重要. 中子衍射揭示了酸合成酶中的位,澄清了PLP.

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相关概念视频

X-ray Crystallography02:18

X-ray Crystallography

The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
Determination of Crystal Structures01:29

Determination of Crystal Structures

In the late 1800s, the revelation that light extended beyond visible wavelengths led to the discovery of X-rays by Wilhelm Roentgen. Recognized as high-energy electromagnetic radiation with short wavelengths, X-rays prompted exploration into their interaction with crystals. Max von Laue proposed in 1912 that the periodic arrangement of atoms, ions, or molecules in crystals would cause them to diffract X-rays, a hypothesis confirmed through experiments with copper sulfate and zinc sulfide...