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Updated: Jun 28, 2025

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
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用于鉴定万科米辛结合蛋白的迪亚齐林光探针

Photis Rotsides1, Paula J Lee1, Nakoa Webber1

  • 1Department of Biochemistry & Molecular Biology and Department of Microbiology & Immunology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102, United States.

ACS bio & med chem Au
|April 22, 2024
PubMed
概括

开发了新的万科米辛光效探针,以识别抗生素结合蛋白. 这些探针提供了增强的特异性和简化方法来检测万科米辛相互作用和抗药机制.

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Labeling DNA Probes03:31

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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