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Updated: Jun 28, 2025

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM
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Published on: December 22, 2015

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一个生物物理工具箱可靠的数据采集和处理在集成的强力对焦光显微镜.

Zhaowei Liu1, Edo van Veen1, Humberto Sánchez1

  • 1Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, 2629 HZ Delft, The Netherlands.

ACS photonics
|April 22, 2024
PubMed
概括

这项研究引入了单分子力光光谱学的多功能工具箱,使同时进行机械和成像测量. 综合系统增强了对生物过程的研究,如蛋白质:DNA相互作用.

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Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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