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Updated: Jun 28, 2025

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
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优化了Fc融合蛋白的生产,通过sortase酶酶结合

Kyle D Apley1, Amy D Laflin1, Stephanie N Johnson1

  • 1Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, Kansas 66045, United States.

Industrial & engineering chemistry research
|April 22, 2024
PubMed
概括

排序酶介导结合 (SML) 提供了一种生产Fc融合药物的高效方法. 这项研究证明了SML.

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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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